Thermal gradient chromatography devices and methods of using them

ABSTRACT

Certain configurations described herein are directed to gas chromatography devices. In some instances, the gas chromatography devices may comprise at least one heating device which can be moved along a chromatography column to provide a thermal gradient to the chromatography column. In other instances, the gas chromatography devices may comprise a heating device that can receive a moving chromatography column to provide a thermal gradient to the chromatography column. The gas chromatography devices may be configured as portable devices which can be used to perform remote analyses.

PRIORITY APPLICATION

This application is related to and claims priority to and the benefit ofU.S. Provisional Application No. 62/248,918 filed on Oct. 30, 2015, theentire disclosure of which is hereby incorporated herein by referencefor all purposes.

TECHNOLOGICAL FIELD

Certain embodiments described herein are related to chromatographydevices. More particularly, certain configurations are disclosed thatare directed to heating devices which can provide a thermal gradient toa chromatography column during a chromatographic separation.

BACKGROUND

Chromatography devices can be used to separate two or more componentspresent in a mixture. The components are typically introduced into achromatography system to separate the components based on theirdifferential solubilities between a mobile phase and a stationary phase.

SUMMARY

Certain aspects described herein are directed to chromatography systemswhere one or both of a heating device (or multiple heating devices) anda chromatography column can be moved to provide a thermal gradient tothe chromatography column during a chromatographic analysis.

In one aspect, a chromatography system comprises a heating deviceconfigured to thermally couple to an inlet section of a chromatographycolumn in a first position and to thermally couple to an exit section ofa chromatography column in a second position, the heating deviceconfigured to move from the first position to the second position duringa chromatographic separation, and a processor configured to controlmovement of the heating device from the first position to the secondposition during the chromatographic separation using the heating deviceto provide a thermal gradient to the chromatography column during thechromatographic separation.

In certain configurations, the system comprises a motor coupled to theheating device and electrically coupled to the processor, the motorconfigured to move the heating device from the first position to thesecond position. In some configurations, the system comprises a DC powersource electrically coupled to the processor. In other examples, the DCpower source comprises at least one of an electrochemical cell, a fuelcell, a solar cell or a wind turbine configured to provide a directcurrent. In certain instances, the system comprises a displayelectrically coupled to the processor. In other examples, the motor isconfigured as a stepper motor. In certain instances, the systemcomprises a transmitter electrically coupled to the processor. In someembodiments, the transmitter is configured to wirelessly couple to amobile device. In some instances, the transmitter comprises at least oneof a Bluetooth device, a near field communication device, a WLAN device,a USB device, a RF device, a cellular device, a radio device, asatellite device, or a GPS device. In certain instances, the systemcomprises an oven configured to thermally couple to the chromatographycolumn and to receive the heating device and the chromatography column.In certain examples, the oven is configured to operate at asubstantially constant temperature during the chromatographicseparation. In some embodiments, the heating device is the only heatingdevice present in the chromatography system to provide heat to thechromatography column. In other examples, the system comprises a coolingdevice thermally coupled to the heating device and the chromatographycolumn, the cooling device configured to assist in providing the thermalgradient to the chromatography column. In some examples, the coolingdevice is configured as one or more of a fan, a Peltier cooler, acooling rod and a heatsink. In some examples, the heating device and thecooling device together are configured to provide a linear thermalgradient along a longitudinal dimension of the chromatography columnfrom the inlet section to the exit section. In certain embodiments, thesystem comprises a detector configured to fluidically couple to thechromatography column. In certain examples, the heating device isconfigured as a cylinder configured to move through an interior spaceformed by coiling of the chromatography column. In other examples, thediameter of the cylinder is sized and arranged to receive and contactsurfaces of chromatography column to provide the thermal gradient to thechromatography column. In certain embodiments, the heating device isconfigured to thermally couple to two or more chromatography columnsduring the chromatographic separation to provide a temperature gradientto each of the two or more chromatography columns. In otherconfigurations, the system comprises a second heating device configuredto thermally couple to the inlet section of the chromatography column ina first position and to thermally couple to the exit section of thechromatography column in a second position, the second heating deviceconfigured to comprise a different temperature than a temperature of theheating device during the chromatographic separation.

In another aspect, a system configured to receive a non-electricallyconductive chromatography column in a column space and provide a thermalgradient to the non-electrically conductive chromatography columnpositioned in the column space during a chromatographic separation, thesystem comprises a heating device comprising a body configured tothermally couple to an inlet section of the non-electrically conductivechromatography column, the body comprising an outer surface that isconfigured to contact at least an inner surface of the inlet section ofthe non-electrically conductive chromatography column to thermallycouple the heating device to the inlet section of the non-electricallyconductive chromatography column, a motor coupled to the heating deviceand configured to provide longitudinal movement of the heating devicealong the non-electrically conductive chromatography column, and aprocessor electrically coupled to the motor and configured to controlthe motor and the longitudinal movement of the heating device from theinlet section of the non-electrically conductive chromatography columntoward an exit section of the non-electrically conductive chromatographycolumn during a chromatographic separation using the heating device toprovide a thermal gradient to the non-electrically conductivechromatography column during the chromatographic separation.

In certain examples, the system comprises an AC power sourceelectrically coupled to the motor and the processor. In other examples,the system comprises a DC power source electrically coupled to the motorand the processor, e.g., at least one of an electrochemical cell, a fuelcell, a solar cell or a wind turbine configured to provide a directcurrent. In other configurations, the system comprises a displayelectrically coupled to the processor. In certain examples, the motor isconfigured as a stepper motor. In some embodiments, the system comprisesa transmitter electrically coupled to the processor. In certaininstances, the transmitter is configured to wirelessly couple to amobile device. In other examples, the transmitter comprises at least oneof a Bluetooth device, a near field communication device, a WLAN device,a USB device, a RF device, a cellular device, a radio device, asatellite device, or a GPS device. In some embodiments, the systemcomprises an oven configured to thermally couple to the chromatographycolumn and to receive the heating device and the chromatography column.In certain instances, the oven is configured to operate at asubstantially constant temperature during the chromatographicseparation. In other configurations, the heating device is the onlyheating device present in the chromatography system to provide heat tothe chromatography column. In some embodiments, the system comprises acooling device thermally coupled to the heating device and configured toassist in providing the thermal gradient to the chromatography column,e.g., the cooling device can be configured as one or more of a fan, aPeltier cooler, a cooling rod and a heatsink. In some examples, theheating device and the cooling device together are configured to providea linear thermal gradient along a longitudinal dimension of thechromatography column from the inlet section to the exit section. Inother embodiments, the system comprises a detector configured tofluidically couple to the chromatography column. In certain instances,the heating device is configured as a cylindrical jacket that contactsinner surfaces of the inlet section of the chromatography column. Insome examples, the heating device is sized and arranged to contact innercoil surfaces of a capillary column coil to provide the thermal gradientto the capillary column. In some embodiments, the heating device isconfigured to thermally couple to two or more chromatography columnsduring the chromatographic separation to provide a temperature gradientto each of the two or more chromatography columns. In other examples,the system comprises a second heating device configured to thermallycouple to the inlet section of the chromatography column in a firstposition and to thermally couple to the exit section of thechromatography column in a second position, the second heating deviceconfigured to comprise a different temperature than a temperature of theheating device during the chromatographic separation

In an additional aspect, a chromatography system comprises a heatingdevice configured to thermally couple to and contact a section of achromatography column at a first position that is less than an overalllength of the chromatography column, and a motor coupled to the heatingdevice and configured to control longitudinal movement of the heatingdevice along the chromatography column from the first position to asecond position different from the first position during achromatographic separation using the heating device to provide a thermalgradient to the chromatography column during the chromatographicseparation.

In certain examples, the system comprises a processor configured tocontrol the motor and longitudinal movement of the heating device duringthe chromatographic separation. In other embodiments, the systemcomprises an AC power source or a DC power source electrically coupledto the processor. In certain instances, the DC power source comprises atleast one of an electrochemical cell, a fuel cell, a solar cell or awind turbine configured to provide a direct current. In other examples,the system comprises a display electrically coupled to the processor. Insome embodiments, the motor is configured as a stepper motor. In certainexamples, the system comprises a transmitter electrically coupled to theprocessor. In some instances, the transmitter is configured towirelessly couple to a mobile device. In certain examples, thetransmitter comprises at least one of a Bluetooth device, a near fieldcommunication device, a WLAN device, a USB device, a RF device, acellular device, a radio device, a satellite device, or a GPS device. Insome embodiments, the system comprises an oven configured to thermallycouple to the chromatography column and to receive the heating deviceand the chromatography column. In other examples, the oven is configuredto operate at a substantially constant temperature during thechromatographic separation. In some embodiments, the heating device isthe only heating device present in the chromatography system to provideheat to the chromatography column. In certain configurations, the systemcomprises a cooling device thermally coupled to the heating device andconfigured to assist in providing the thermal gradient to thechromatography column. In some examples, the cooling device isconfigured as one or more of a fan, a Peltier cooler, a cooling rod anda heat sink. In other examples, the heating device and the coolingdevice together are configured to provide a linear thermal gradientalong a longitudinal dimension of the chromatography column from thefirst position to the second position. In some embodiments, the systemcomprises a detector configured to fluidically couple to thechromatography column. In other embodiments, the heating device isconfigured as a cylindrical jacket that contacts inner coil surfaces ofthe inlet section of the chromatography column. In some examples, theheating device is sized and arranged to contact inner coil surfaces of acapillary column coil to provide the thermal gradient to the capillarycolumn. In some embodiments, the heating device is configured tothermally couple to two or more chromatography columns during thechromatographic separation to provide a temperature gradient to each ofthe two or more chromatography columns. In other configurations, thesystem comprises a second heating device configured to thermally coupleto and contact the section of a chromatography column at a firstposition that is less than an overall length of the chromatographycolumn, the second heating device configured to comprise a differenttemperature than a temperature of the heating device during thechromatographic separation.

In another aspect, a kit comprises a heating device configured tothermally couple to a chromatography column at a first position, andinstructions for using the heating device with the chromatography columnto provide a thermal gradient to the chromatography column during achromatographic separation by moving the heating device in alongitudinal direction along the chromatography column from the firstposition to a second position different from the first position.

In certain configurations, the kit comprises a motor configured tocouple to the heating device to move the heating device in thelongitudinal direction. In other configurations, the motor is a steppermotor. In some embodiments, the kit comprises a processor configured toelectrically couple to the motor and control movement of the heatingdevice in the longitudinal direction. In some examples, the kitcomprises a chromatography column configured for use with the heatingdevice. In some examples, the chromatography column is selected from thegroup consisting of a coiled capillary column, a coiled capillary columnbundle, a wafer column and a non-coiled capillary column. In otherexamples, the kit comprises a DC power source. In some embodiments, theDC power source comprises at least one of an electrochemical cell, afuel cell, a solar cell or a wind turbine configured to provide a directcurrent. In some instances, the kit comprises a detector. In someembodiments, the kit comprises a second DC power source different fromthe DC power source.

In another aspect, a method comprises providing a heating deviceconfigured to thermally couple to a chromatography column at an inletsection of the chromatography column in a first position and tothermally couple to the chromatography column at a section downstreamfrom the inlet section in a second position, the heating deviceconfigured to move in a longitudinal dimension along the chromatographycolumn from the first position to the second position during achromatographic separation to provide a thermal gradient during thechromatographic separation.

In certain instances, the method comprises providing a substantiallylinear thermal gradient during the chromatographic separation bymaintaining the heating device at a substantially constant temperatureduring the chromatographic separation. In other instances, the methodcomprises providing a substantially linear thermal gradient during thechromatographic separation using a cooling device thermally coupled tothe heating device. In some embodiments, the method comprises moving thecooling device during the chromatographic separation. In certainexamples, the method comprises maintaining the cooling device in astationary position during the chromatographic separation. In someinstances, the method comprises moving the heating device from the firstposition to the second position using a motor coupled to the heatingdevice. In some embodiments, the method comprises providing power to themotor using a DC power source electrically coupled to the motor. Incertain examples, the method comprises providing a non-linear thermalgradient using the heating device. In some examples, the methodcomprises configuring the chromatography system with a processor. Incertain embodiments, the method comprises configuring the processor towirelessly couple to a mobile device that receives chromatographyinformation from the system during the chromatographic separation.

In another aspect, a method of performing gas chromatography comprisesproviding a heating device configured to thermally couple to inner coilsurfaces of a section of a chromatography column coil in a firstposition of the heating device and configured to thermally couple toinner surfaces of a different section of the chromatography column coilin a second position, the heating device configured to move from thefirst position to the second position to provide a thermal gradientduring a gas chromatographic separation.

In some instances, the method comprises providing a cooling deviceconfigured to thermally couple to the heating device, the heating deviceand cooling device together configured to provide a substantially linearthermal gradient during the gas chromatographic separation. In otherinstances, the method comprises providing the substantially linearthermal gradient during the chromatographic separation by maintainingone end of the heating device at a substantially constant temperatureduring the chromatographic separation. In certain examples, the methodcomprises moving the cooling device during the chromatographicseparation. In some embodiments, the method comprises maintaining thecooling device in a stationary position during the chromatographicseparation. In some examples, the method comprises moving the heatingdevice from the first position to the second position using a motorcoupled to the heating device. In certain examples, the method comprisesproviding power to the motor using a DC power source electricallycoupled to the motor. In some examples, the method comprises providing anon-linear thermal gradient using the heating device. In certainexamples, the method comprises configuring the chromatography systemwith a processor. In some embodiments, the method comprises configuringthe processor to wireles sly couple to a mobile device that receiveschromatography information from the system during the chromatographicseparation.

In an additional aspect, a method of separating two or more analytesusing a thermal gradient comprises providing a heating device configuredto thermally couple to a chromatography column in a column space of achromatography system, in which the heating device is configured tothermally couple to the inlet section of the chromatography column in afirst position and to thermally couple to a section of thechromatography column downstream of the inlet section in a secondposition, and providing instructions for moving the heating device in alongitudinal direction of the chromatography column from the firstposition to the second position to provide a thermal gradient to thechromatography column during a chromatographic separation.

In certain examples, the method comprises providing a chromatographycolumn configured to receive the heating device in an inner space formedby coiling of the chromatography column. In some embodiments, the methodcomprises providing a motor configured to couple to the heating deviceto control movement of the heating device in the longitudinal direction.In other examples, the method comprises providing a cooling deviceconfigured to thermally couple to a section of the chromatography columnother than a section where the heating device is thermally coupled, inwhich the heating device and cooling device together are configured toprovide a substantially linear thermal gradient during chromatographicseparation using the chromatography column. In some instances, themethod comprises configuring the cooling device as a fan.

In another aspect, a portable chromatography system comprises a housingsized and arranged to permit carrying of the system by a human, thehousing comprising, an injector, a column space configured to receive achromatography column that fluidically couples to the injector toprovide sample injected into the injector to an inlet section of thechromatography column, a heating device configured to thermally coupleto the chromatography column in the column space, in which the heatingdevice is configured to thermally couple to the inlet section of thechromatography column in a first position and to thermally couple to asection of the chromatography column downstream of the inlet section ina second position, in which the heating device is configured to move ina longitudinal direction of the chromatography column from the firstposition to the second position to provide a thermal gradient duringchromatographic separation using the heating device, a detectorconfigured to fluidically couple to the chromatography column at an exitend of the chromatography column to receive analyte from thechromatography column, and a processor electrically coupled to thedetector.

In certain configurations, the system comprises a motor within thehousing and coupled to the heating device and electrically coupled tothe processor. In other configurations, the system comprises a DC powersource electrically coupled to the processor and positioned within thehousing. In some embodiments, the DC power source is configured as anelectrochemical cell, a fuel cell, a solar cell or a wind turbineconfigured to provide a direct current. In other instances, the heatingdevice is configured to thermally couple to a column that is one or moreof a capillary column, a capillary column bundle and a wafer column.

In another aspect, a chromatography system comprises a heating deviceconfigured to thermally couple to an inlet section of a chromatographycolumn in a first position and to thermally couple to an exit section ofa chromatography column in a second position, and a processor configuredto control movement of the chromatography column from the first positionto the second position during the chromatographic separation using theheating device to provide a thermal gradient to the chromatographycolumn during the chromatographic separation.

In certain configurations, the system comprises a motor coupled to thechromatography column device and electrically coupled to the processor,the motor configured to move the chromatography column from the firstposition to the second position. In other configurations, the systemcomprises a DC power source electrically coupled to the processor. Insome embodiments, the DC power source comprises at least one of anelectrochemical cell, a fuel cell, a solar cell or a wind turbineconfigured to provide a direct current. In certain examples, the systemcomprises a display electrically coupled to the processor. In otherexamples, the motor is configured as a stepper motor. In someembodiments, the system comprises a transmitter electrically coupled tothe processor. In certain examples, the transmitter is configured towirelessly couple to a mobile device. In some examples, the transmittercomprises at least one of a Bluetooth device, a near field communicationdevice, a WLAN device, a USB device, a RF device, a cellular device, aradio device, a satellite device, or a GPS device. In certainembodiments, the system comprises an oven configured to thermally coupleto the chromatography column and to receive the heating device and thechromatography column. In other embodiments, the oven is configured tooperate at a substantially constant temperature during thechromatographic separation. In some examples, the heating device is theonly heating device present in the chromatography system to provide heatto the chromatography column. In other examples, the system comprises acooling device thermally coupled to the heating device and thechromatography column, the cooling device configured to assist inproviding the thermal gradient to the chromatography column. In someembodiments, the cooling device is configured as one or more of a fan, aPeltier cooler, a cooling rod and a heatsink. In certain examples, theheating device and the cooling device together are configured to providea linear thermal gradient along a longitudinal dimension of thechromatography column from the inlet section to the exit section. Insome embodiments, the system comprises a detector configured tofluidically couple to the chromatography column. In some examples, thesystem comprises a motor coupled to the heating device, the motorconfigured to move the heating device during movement of thechromatography column. In other examples, the heating device is sizedand arranged to receive and contact surfaces of chromatography column toprovide the thermal gradient to the chromatography column. In someexamples, the heating device is configured to thermally couple to two ormore chromatography columns during the chromatographic separation toprovide a temperature gradient to each of the two or more chromatographycolumns. In certain embodiments, the system comprises a second heatingdevice configured to thermally couple to the inlet section of thechromatography column in a first position and to thermally couple to theexit section of the chromatography column in a second position, thesecond heating device configured to comprise a different temperaturethan a temperature of the heating device during the chromatographicseparation.

In an additional aspect, a system configured to receive anon-electrically conductive chromatography column in a column space andprovide a thermal gradient to the non-electrically conductivechromatography column positioned in the column space during achromatographic separation comprises a heating device comprising a bodyconfigured to thermally couple to an inlet section of thenon-electrically conductive chromatography column, the body comprisingan outer surface that is configured to contact at least an inner coilsurface of the inlet section of the non-electrically conductivechromatography column coil to thermally couple the heating device to theinlet section of the non-electrically conductive chromatography columncoil, a motor configured to couple to the non-electrically conductivechromatography column coil to provide longitudinal movement of thenon-electrically conductive chromatography column coil along the heatingdevice, and a processor electrically coupled to the motor and configuredto control the motor and the longitudinal movement of thenon-electrically conductive chromatography column coil from the inletsection of the non-electrically conductive chromatography column coiltoward an exit section of the non-electrically conductive chromatographycolumn coil during a chromatographic separation using the heating deviceto provide a thermal gradient to the non-electrically conductivechromatography column coil during the chromatographic separation. Ifdesired, the system can be configured for use with non-coil columns aswell.

In certain configurations, the system comprises an AC power sourceelectrically coupled to the motor and the processor. In someembodiments, the system comprises a DC power source electrically coupledto the motor and the processor. In certain examples, the DC power sourcecomprises at least one of an electrochemical cell, a fuel cell, a solarcell or a wind turbine configured to provide a direct current. In otherexamples, the system comprises a display electrically coupled to theprocessor. In some embodiments, the motor is configured as a steppermotor. In certain embodiments, the system comprises a transmitterelectrically coupled to the processor. In other embodiments, thetransmitter is configured to wirelessly couple to a mobile device. Insome examples, the transmitter comprises at least one of a Bluetoothdevice, a near field communication device, a WLAN device, a USB device,a RF device, a cellular device, a radio device, a satellite device, or aGPS device. In certain configurations, the system comprises an ovenconfigured to thermally couple to the chromatography column coil and toreceive the heating device and the chromatography column coil. In otherconfigurations, the oven is configured to operate at a substantiallyconstant temperature during the chromatographic separation. In someembodiments, the heating device is the only heating device present inthe chromatography system to provide heat to the chromatography column.In other embodiments, the system comprises a cooling device thermallycoupled to the heating device and configured to assist in providing thethermal gradient to the chromatography column coil. In certain examples,the cooling device is configured as one or more of a fan, a Peltiercooler, a cooling rod and a heatsink. In some instances, the heatingdevice and the cooling device together are configured to provide alinear thermal gradient along a longitudinal dimension of thechromatography column coil from the inlet section to the exit section.In other instances, the system comprises a detector configured tofluidically couple to the chromatography column. In some embodiments,the system comprises a second motor coupled to the heating device, themotor configured to move the heating device during movement of thechromatography column coil. In certain examples, the heating device issized and arranged to contact inner coil surfaces of a capillary columncoil to provide the thermal gradient to the capillary column coil. Insome examples, the heating device is configured to thermally couple totwo or more chromatography columns during the chromatographic separationto provide a temperature gradient to each of the two or morechromatography columns. In certain configurations, the system comprisesa second heating device configured to thermally couple to the inletsection of the chromatography column in a first position and tothermally couple to the exit section of the chromatography column in asecond position, the second heating device configured to comprise adifferent temperature than a temperature of the heating device duringthe chromatographic separation.

In another aspect, a chromatography system comprises a heating deviceconfigured to thermally couple to and contact a section of achromatography column at a first position that is less than an overalllength of the chromatography column, and a motor configured to couple tothe chromatography column and configured to control longitudinalmovement of the chromatography column along the heating device from thefirst position to a second position different from the first positionduring a chromatographic separation using the heating device to providea thermal gradient to the chromatography column during thechromatographic separation.

In some examples, the system comprises a processor configured to controlthe motor and longitudinal movement of the chromatography column duringthe chromatographic separation. In certain instances, the systemcomprises a DC power source electrically coupled to the processor. Insome examples, the DC power source comprises at least one of anelectrochemical cell, a fuel cell, a solar cell or a wind turbineconfigured to provide a direct current. In other examples, the systemcomprises a display electrically coupled to the processor. In someconfigurations, the motor is configured as a stepper motor. In otherexamples, the system comprises a transmitter electrically coupled to theprocessor. In some examples, the transmitter is configured to wirelessly couple to a mobile device. In other instances, the transmittercomprises at least one of a Bluetooth device, a near field communicationdevice, a WLAN device, a USB device, a RF device, a cellular device, aradio device, a satellite device, or a GPS device. In some examples, thesystem comprises an oven configured to thermally couple to thechromatography column and to receive the heating device and thechromatography column. In some embodiments, the oven is configured tooperate at a substantially constant temperature during thechromatographic separation. In other examples, the heating device is theonly heating device present in the chromatography system to provide heatto the chromatography column. In some configurations, the systemcomprises a cooling device thermally coupled to the heating device andconfigured to assist in providing the thermal gradient to thechromatography column. In other instances, the cooling device isconfigured as one or more of a fan, a Peltier cooler, a cooling rod anda heat sink. In some embodiments, the heating device and the coolingdevice together are configured to provide a linear thermal gradientalong a longitudinal dimension of the chromatography column from thefirst position to the second position. In some examples, the systemcomprises a detector configured to fluidically couple to thechromatography column. In other examples, the system comprises a secondmotor coupled to the heating device, the second motor configured to movethe heating device during movement of the chromatography column. In someembodiments, the heating device is sized and arranged to contact innercoil surfaces of a capillary column coil to provide the thermal gradientto the capillary column coil. In other configurations, the heatingdevice is configured to thermally couple to two or more chromatographycolumns during the chromatographic separation to provide a temperaturegradient to each of the two or more chromatography columns. In someembodiments, the system comprises a second heating device configured tothermally couple to and contact the section of a chromatography columnat a first position that is less than an overall length of thechromatography column, the second heating device configured to comprisea different temperature than a temperature of the heating device duringthe chromatographic separation.

In an additional aspect, a kit comprises a heating device configured tothermally couple to a chromatography column at a first position, andinstructions for using the heating device with the chromatography columnto provide a thermal gradient to the chromatography column during achromatographic separation by moving the chromatography column in alongitudinal direction along the heating device from the first positionto a second position different from the first position.

In some instances, the kit comprises a motor configured to couple to thechromatography column to move the chromatography column in thelongitudinal direction. In other instances, the motor is a steppermotor. In some examples, the kit comprises a processor configured toelectrically couple to the motor and control movement of thechromatography column in the longitudinal direction. In someembodiments, the kit comprises a chromatography column configured foruse with the heating device. In certain examples, the chromatographycolumn is selected from the group consisting of a coiled capillarycolumn, a coiled capillary column bundle, a wafer column and anon-coiled capillary column. In some embodiments, the kit comprises a DCpower source. In some examples, the DC power source comprises at leastone of an electrochemical cell, a fuel cell, a solar cell or a windturbine configured to provide a direct current. In some instances, thekit comprises a detector. In other examples, the kit comprises a secondDC power source different from the DC power source.

In another aspect, a method comprises providing a heating deviceconfigured to thermally couple to a chromatography column at an inletsection of the chromatography column in a first position and tothermally couple to the chromatography column at a section downstreamfrom the inlet section in a second position, the heating deviceconfigured to receive the chromatography column during movement of thechromatography column in a longitudinal dimension along the heatingdevice from the first position to the second position during achromatographic separation to provide a thermal gradient to thechromatography column during the chromatographic separation.

In certain examples, the method comprises providing a substantiallylinear thermal gradient during the chromatographic separation bymaintaining the heating device at a substantially constant temperatureduring the chromatographic separation. In other examples, the methodcomprises providing a substantially linear thermal gradient during thechromatographic separation using a cooling device thermally coupled tothe heating device. In some embodiments, the method comprises moving thecooling device during the chromatographic separation. In certainexamples, the method comprises maintaining the cooling device in astationary position during the chromatographic separation. In someinstances, the method comprises moving the chromatography column fromthe first position to the second position using a motor coupled to thechromatography column. In some embodiments, the method comprisesproviding power to the motor using a DC power source electricallycoupled to the motor. In certain examples, the method comprisesproviding a non-linear thermal gradient using the heating device. Inother examples, the method comprises configuring the chromatographysystem with a processor. In some examples, the method comprisesconfiguring the processor to wirelessly couple to a mobile device thatreceives chromatography information from the system during thechromatographic separation.

In an additional aspect, a method of performing gas chromatographycomprises providing a heating device configured to thermally couple toinner coil surfaces of a section of a chromatography column coil in afirst position of the heating device and configured to thermally coupleto inner coil surfaces of a different section of the chromatographycolumn coil in a second position, the heating device configured toreceive the chromatography column coil during movement of thechromatography column coil from the first position to the secondposition to provide a thermal gradient to the chromatography column coilduring a gas chromatographic separation.

In certain examples, the method comprises providing a cooling deviceconfigured to thermally couple to the heating device, the heating deviceand cooling device together configured to provide a substantially linearthermal gradient during the gas chromatographic separation. In otherexamples, the method comprises providing the substantially linearthermal gradient during the chromatographic separation by maintainingone end of the heating device at a substantially constant temperatureduring the chromatographic separation. In some instances, the methodcomprises moving the cooling device during the chromatographicseparation. In other examples, the method comprises maintaining thecooling device in a stationary position during the chromatographicseparation. In certain instances, the method comprises moving thechromatography column coil from the first position to the secondposition using a motor coupled to the heating device. In other examples,the method comprises providing power to the motor using a DC powersource electrically coupled to the motor. In certain examples, themethod comprises providing a non-linear thermal gradient using theheating device. In some embodiments, the method comprises configuringthe chromatography system with a processor. In some instances, themethod comprises configuring the processor to wirelessly couple to amobile device that receives chromatography information from the systemduring the chromatographic separation.

In another aspect, a method of separating two or more analytes using athermal gradient, comprises providing a heating device configured tothermally couple to a chromatography column in a column space of achromatography system, in which the heating device is configured tothermally couple to the inlet section of the chromatography column in afirst position and to thermally couple to a section of thechromatography column downstream of the inlet section in a secondposition, and providing instructions for moving the chromatographycolumn in a longitudinal direction of the chromatography column from thefirst position to the second position to provide a thermal gradient tothe chromatography column during a chromatographic separation.

In certain examples, the method comprises providing a chromatographycolumn configured to receive the heating device in an inner space formedby coiling of the chromatography column. In some embodiments, the methodcomprises providing a motor configured to couple to the chromatographycolumn to control movement of the chromatography column in thelongitudinal direction. In certain examples, the method comprisesproviding a cooling device configured to thermally couple to a sectionof the chromatography column other than a section where the heatingdevice is thermally coupled, in which the heating device and coolingdevice together are configured to provide a substantially linear thermalgradient during chromatographic separation using the chromatographycolumn. In some embodiments, the method comprises configuring thecooling device as a fan.

In another aspect, a portable chromatography system comprises a housingsized and arranged to permit carrying of the system by a human, thehousing comprising an injector, a column space configured to receive achromatography column that fluidically couples to the injector toprovide sample injected into the injector to an inlet section of thechromatography column, a heating device configured to thermally coupleto the chromatography column in the column space, in which the heatingdevice is configured to thermally couple to the inlet section of thechromatography column in a first position and to thermally couple to asection of the chromatography column downstream of the inlet section ina second position, in which the heating device is configured to receivethe chromatography column during movement of the chromatography columnin a longitudinal direction from the first position to the secondposition to provide a thermal gradient during chromatographic separationusing the heating device, a detector configured to fluidically couple tothe chromatography column at an exit end of the chromatography column toreceive analyte from the chromatography column, and a processorelectrically coupled to the detector.

In some examples, the system comprises a motor within the housing andcoupled to the chromatography column and electrically coupled to theprocessor. In other examples, the system comprises a DC power sourceelectrically coupled to the processor and positioned within the housing.In certain examples, the DC power source is configured as anelectrochemical cell, a fuel cell, a solar cell or a wind turbineconfigured to provide a direct current. In some examples, the heatingdevice is configured to thermally couple to a column that is one or moreof a capillary column, a capillary column bundle and a wafer column.

In another aspect, a method comprises providing a first heating deviceand a second heating device each configured to thermally couple to achromatography column at an inlet section of the chromatography columnin a first position and to thermally couple to the chromatography columnat a section downstream from the inlet section in a second position,each of the first heating device and the second heating deviceconfigured to move in a longitudinal dimension along the chromatographycolumn from the first position to the second position during achromatographic separation to provide a thermal gradient during thechromatographic separation, in which the first heating device isconfigured to thermally couple to the column for a first period and thesecond heating device is configured to thermally couple to the columnafter the first period once the first heating device has been thermallydecoupled from the column.

In certain examples, the method comprises providing a substantiallylinear thermal gradient during the chromatographic separation bymaintaining the first and second heating devices at a substantiallyconstant temperature during the chromatographic separation. In otherexamples, the method comprises providing a substantially linear thermalgradient during the chromatographic separation using a cooling devicethermally coupled to at least one of the first and second heatingdevices. In some examples, the method comprises moving the coolingdevice during the chromatographic separation. In certain embodiments,the method comprises maintaining the cooling device in a stationaryposition during the chromatographic separation. In some examples, themethod comprises moving each of the first and second heating devicesfrom the first position to the second position using a motor coupled tothe heating device. In certain examples, the method comprises providingpower to the motor using a DC power source electrically coupled to themotor. In some embodiments, the method comprises providing a non-linearthermal gradient using the first and second heating devices. In certainembodiments, the method comprises configuring the chromatography systemwith a processor. In other instances, the method comprises configuringthe processor to wirelessly couple to a mobile device that receiveschromatography information from the system during the chromatographicseparation.

In an additional aspect, a method of performing gas chromatographycomprises providing a first heating device and a second heating deviceeach configured to thermally couple to inner coil surfaces of a sectionof a chromatography column coil in a first position of each of the firstand second heating devices and configured to thermally couple to innercoil surfaces of a different section of the chromatography column coilin a second position, the first and second heating devices configured tomove from the first position to the second position to provide a thermalgradient during a gas chromatographic separation, in which the firstheating device is configured to thermally couple to the section for afirst period and the second heating device is configured to thermallycouple to the section after the first period and once the first heatingdevice has been thermally decoupled from the section.

In certain configurations, the method comprises providing a coolingdevice configured to thermally couple to one of the first and secondheating devices, the first and second heating devices and the coolingdevice together configured to provide a substantially linear thermalgradient during the gas chromatographic separation. In other examples,the method comprises providing the substantially linear thermal gradientduring the chromatographic separation by maintaining one end of thefirst and second heating devices at a substantially constant temperatureduring the chromatographic separation. In some examples, the methodcomprises moving the cooling device during the chromatographicseparation. In some embodiments, the method comprises maintaining thecooling device in a stationary position during the chromatographicseparation. In other examples, the method comprises moving the first andsecond heating devices from the first position to the second positionusing a motor coupled to the first and second heating devices. Incertain instances, the method comprises providing power to the motorusing a DC power source electrically coupled to the motor. In someembodiments, the method comprises providing a non-linear thermalgradient using the first and second heating devices. In certainexamples, the method comprises configuring the chromatography systemwith a processor. In other examples, the method comprises configuringthe processor to wirelessly couple to a mobile device that receiveschromatography information from the system during the chromatographicseparation.

Additional features, aspect, examples, configurations and embodimentsare described in more detail below.

BRIEF DESCRIPTION OF THE FIGURES

Certain embodiments are described with reference to the accompanyingfigures in which:

FIGS. 1A-6B are illustrations showing a heating device, column and athermal gradient as the heating device is moved along a longitudinaldimension of the column, in accordance with certain examples;

FIG. 7 is an illustration of a heating device comprising differentsections, in accordance with certain examples;

FIG. 8 is an illustration of a heating device configured to contactouter surfaces of a coiled column, in accordance with certainembodiments;

FIG. 9 is an illustration of a heating device configured to contactinner and outer surfaces of a coiled column, in accordance with certainembodiments;

FIG. 10 is an illustration of a heating device configured for use with awafer column, in accordance with certain examples;

FIG. 11 is another illustration of a heating device configured for usewith a wafer column, in accordance with certain examples;

FIG. 12 is an illustration of a heating device comprising apertures atone end of the heating device, in accordance with certainconfigurations;

FIG. 13 is an illustration of a heating device that can be usedsimultaneously with two coiled columns, in accordance with certainexamples;

FIG. 14 is an illustration of a heating device that can be usedsimultaneously with two wafer columns, in accordance with certainexamples;

FIG. 15 is another illustration of a heating device that can be usedwith two coiled columns, in accordance with certain embodiments;

FIG. 16A is an illustration of a chromatography system comprising amotor, a heating device and a chromatography column, in accordance withcertain examples;

FIG. 16B is an illustration of a chromatography system comprising aprocessor, a motor, a heating device and a chromatography column, inaccordance with certain examples;

FIG. 17 is an illustration of a gas chromatography system comprising aninjector, an oven, a heating device, a column and a detector, inaccordance with certain configurations;

FIG. 18 is a block diagram showing a system comprising a gaschromatography device coupled to a mass spectrometer, in accordance withcertain examples;

FIG. 19 is an illustration of a portable gas chromatography device, inaccordance with certain embodiments;

FIG. 20 is an illustration of a system comprising first and secondheating devices, in accordance with certain configurations;

FIG. 21A is an illustration showing insertion of a first heating deviceinto an interior portion of a column, in accordance with certainexamples;

FIG. 21B is an illustration showing insertion of a second heating deviceinto an interior portion of a column, in accordance with certainexamples;

FIG. 22 is an illustration showing two heating devices where one heatingdevice is sued to retain a column, in accordance with certainembodiments;

FIG. 23 is a graph showing temperature measurements along a length of acolumn using a single heating device, in accordance with certainexamples; and

FIG. 24 is a graph showing temperature measurements along a length of acolumn using two heating devices, in accordance with certain examples.

It will be recognized by the person of ordinary skill in the art, giventhe benefit of this disclosure, that certain dimensions or features inthe figures may have been enlarged, distorted or shown in an otherwiseunconventional or non-proportional manner to provide a more userfriendly version of the figures. No particular length, diameter orthickness, is intended by the depictions in the figures, and relativesizes of the figure components are not intended to limit the sizes ofany of the components in the figures. Where dimensions or values arespecified in the description below, the dimensions or values areprovided for illustrative purposes only.

DETAILED DESCRIPTION

Certain embodiments are described below with reference to singular andplural terms in order to provide a more user friendly description of thetechnology disclosed herein. These terms are used for conveniencepurposes only and are not intended to limit the chromatography systems,heating devices and their use as including or excluding certain featuresunless otherwise noted as being present in a particular embodimentdescribed herein. Certain devices are described herein as beingthermally coupled to each other. Thermal coupling refers to the abilityof heat to transfer from one component to another component. Othercomponents herein may be fluidically coupled to each other, which refersto the ability of a fluid such as a liquid or gas being able to flowfrom one component to another component. The chromatography devicesdescribed herein may be configured to receive a liquid or gas sample (orboth) and separate components in the sample using a chromatographycolumn and the heating device.

In certain configurations of the devices and systems described herein, aheating device or heater may be present to provide a temperaturegradient. The heating device need not be the only heating devicepresent, and in many configurations, the heating device is configured asa secondary heating device that can provide a thermal gradient. Forexample, the device or system may comprise a first heating device suchas an oven to provide a substantially constant temperature and caninclude a second heating device that can provide a thermal gradient. Theheating device that provides the thermal gradient may be configured as alow power device to permit the devices and system including the heatingdevice to be portable and/or use power from DC power sources such asbatteries, fuel cells, wind generators and/or photovoltaic cells.

In some instances, the exact nature of the thermal gradient provided bythe heating device may vary. In certain configurations, the heatingdevice can be configured to move or translate along another device,e.g., a column. For example, a column can be placed within the heatingdevice, the heating device can be placed within an inner space formed bythe column, the heating device can be placed adjacent to a column or theheating device may otherwise be thermally coupled to the column suchthat a thermal gradient exists in the portion of the column thermallycoupled to the heating device. In some configurations, one end of theheating device may comprise a higher temperature than another end of theheating device to provide the thermal gradient. For example, a fan,heatsink Peltier cooler, a cooling rod or the like can be thermallycoupled or physically contact one end of the heating device to provide atemperature different from one end of the heating device to the otherend of the heating device. The thermal gradient may be linear,non-linear or may take other forms or shapes as desired. The exact speedin which the heating device is moved from one portion of a column toanother portion of a column (or vice versa when the column is moved) canvary and may assist in controlling the shape of the thermal gradient. Insome embodiments, the heating device may be in direct contact with oneor more surfaces of a column, whereas in other examples a small air gapor other intervening material or gas may be present between the heatingdevice and the column surface. Where the column takes the form of acoil, the heating device may be moved through the inner space formed bythe coil from an inlet section of the coiled column toward an outletsection of the coiled column. This longitudinal movement of the heatingdevice from an inlet section toward an outlet section incrementallyprovides a thermal gradient to different sections of the coiled column.In certain instances, the heating device may take the form of one ormore cylinders that can be moved along the longitudinal axis of theheating device and into and/or out of a coiled column. As noted herein,the coiled column typically resides in oven that provides fortemperature control during a chromatographic separation.

To illustrate the basic operation of the heating device, FIGS. 1A-6Billustrate movement of a heating device from an inlet section of acolumn toward an outlet section of a column. Referring to FIG. 1B, adevice is shown that comprises a heating device 110 and a column 120surrounded by insulation or an insulating member 130. The column 120 isshown in a side view and typically is coiled to decrease the overallcolumn space that the column will occupy. For example, the overalllength of the column 120 may be 1 meter or more, 10 meters or more,etc., and coiling of the column 120 can reduce the overall space withinthe device or system including the column 120. The column 120 comprisesan inlet or inlet section 122 and an exit or exit section 134. The inletsection 122 is typically fluidically coupled to an injector or othersample introduction device (not shown) to permit sample to be introducedinto the column 120. The exit section 124 may be fluidically coupled toa detector (not shown) to provide separated analyte components to thedetector for detection. The heating device 110 and column 120 typicallyare present within an oven (not shown) to maintain the column 120 at asubstantially constant temperature, though the oven temperature can beadjusted during the separation if desired. Referring to FIG. 1A, thetemperature of the heating device along the length of the heating deviceis shown using curve 160 and the temperature of the column along thelength of the column is shown using curve 170 at an initial or startingposition of the heating device 110. As shown in curve 160, the heatingdevice 110 has a thermal gradient from one side of the heating device110 to the other. For example, the temperature of the heating devicenear an end 112 can be higher than the temperature of the heating device110 near an opposite end 114. In some instances, the temperature of theheating device 110 at the end 114 may be about the same temperature asthe oven temperature so that the inlet section 122 of the column 120 isheld at about the same temperature as the oven temperature. While alinear thermal gradient is shown in curve 160, if desired the thermalgradient provided by the heating device 110 may be non-linear. Coolingair can be provided in the direction of arrows 150 to keep thetemperature of the end 114 of the heating device 110 less than atemperature of the end 112 of the heating device 110. In alternativeconfigurations described below, the temperature of individual sectionsor portions of the heating device can be controlled to provide a desiredthermal gradient. Different analytes components are shown as sandwichedanalyte peaks 180 in FIG. 1A with no baseline separation between thedifferent analytes.

In use of the heating device 110, the heating device 110 can beconfigured as a cylinder that can be inserted into and out of innerspace formed by coiling of a capillary column. The heating device 110can be heated at the end 112 by a rod held at constant temperature(upper temperature limit for the desired separation) or may be heated inother configurations as noted herein. The opposite end of the cylindercan be cooled using the air 150 or using a heatsink, cooling rod orPeltier cooler coupled to the end 114. The combination of the heat andcooling can provide a thermal gradient along the length of the cylinderfrom the end 112 to the end 114. The longitudinal gaps between each coilof the column 120 can be spaced large to small from the end 122 to theend 124 (see the spaced circles in FIG. 1B) so that a decaying thermalgradient is provided along the length of the column 120. Alternatively,the column coils may be spaced about the same in a longitudinaldirection, and the thermal gradient can be provided by differentialtemperatures from the heating device. A sample can be introduced at theend 122 of the column 120. The sample can be a complex or simplemixture. For this example, there are 4 analytes in the mixture.

In certain instances, after the sample is introduced to the head 122 ofthe column 120 the components separate out along the column 120 with themore volatile components traveling further to the end 124 of the column120 and the less volatile components remaining closer to the inlet end122 of the column 120 (see FIGS. 2A and 2B). The heating device 110 maythen be moved from its initial position and into the inner space of thecolumn 120 to a different position (see FIGS. 3A and 3B). As shown inthe curve 170 in FIG. 3A, this movement of the heating device 110 altersthe temperature along the length of the column 120. As the heatingdevice 110 is inserted into the column 120, the components on the column120 experience a higher temperature and are eluted out the end 124 ofthe column 120 to a detector (not shown) or other component or device.The speed of insertion of the heating device determines, at least inpart, the speed of elution.

Referring now to FIGS. 4A-6B, continued insertion of the heating device110 toward an exit end 124 of the column 120 is shown. As the end 114 ofheating device 110 gets closer to the end 124 of the column 120, thetemperature of the column 120 increases. Decreased coil spacing at theend 124 of the column 120 causes remaining components to elute quickly.As the heating device 110 nears or becomes adjacent to the end 124 ofthe column 120, all components in the mixture should have eluted fromthe column 120. These components are shown as separated analyte peaks ineach of FIGS. 4A, 5A and 6A. The heating device 110 can then be movedback to its initial position shown in FIGS. 1B and 2B prior tointroduction of another sample into the chromatography device. Ifdesired, the heating device 110 may remain in the position shown in FIG.6B for some period to assist in elution of any remaining components fromthe column 120. Further, the temperature of the heating devicepositioned in FIG. 6B can be increased to assist in elution of any hightemperature components still remaining on the column. In an alternativeconfiguration, the heating devices 110 of FIGS. 1A-6B can remainstationary, and the column 120 may instead be moved. Or, in someinstances, both the heating device 110 and the column 120 can be movedif desired.

In certain configurations, the heating devices described herein may takethe form of a cylinder (which can be solid or hollow). The cylinder maybe electrically coupled to a power source such that application of acurrent to the cylinder causes heating of the cylinder. As noted herein,the cylinder may be configured to provide a first temperature at one endand a second temperature, different from the first temperature, at asecond end such that a thermal gradient exists between the ends of thecylinder. The differential temperature may be provided, for example, byselectively cooling one end of the cylinder using an air current, heatsink, Peltier cooler, cooling rod or other cooling devices or means. Inother instances, the cylinder may comprise individual cylinder sectionswhose temperatures can be individually regulated. One illustration isshown in FIG. 7. The heating device 700 comprises a generallycylindrical body comprising two or more individual sections such assections 710, 720, and 730. The sections 710, 720 and 730 can beseparated by an insulating material or member, e.g., members 715, 725such that current applied to one section does not reach another section.The insulators 715, 725 are desirably thick enough to prevent chargetransfer between sections 710, 720 and 730 but not so thick so as tocreate a temperature drop or cold spot between the sections 710, 720 and730. In the configuration shown in FIG. 7, the electrical couplingsbetween each of the sections 710, 720,730 and a power source may bepresent in an interior portion of the sections 710, 720 and 730 so as tonot interfere with heat transfer from the sections 710, 720 and 730 tothe column (not shown). For example, a power source 750 is shown aselectrically coupled to the section 710 through an interior portion ofthe section 710 to permit movement of the device 700. While not shown,sections 720 and 730 typically comprise their own power source.Alternatively, a resistor network or other devices can be used so that asingle power source can be coupled to the various sections, butdifferent amounts of power can be provided to alter the temperature ofthe various sections 710, 720, 730 of the device 700. In some instances,a sectioned heating device may remain stationary, and the column mayinstead be moved. In other configurations, the sectioned heating deviceand the column may both be moved if desired.

In certain embodiments, the heating device used in FIGS. 1A-6B and shownin FIG. 7 can be used to contact inner coil surfaces of a column byinserting the heating device into open inner space formed by coiling ofthe column. The heating device may physically contact the inner coilsurfaces using grooves or other structures in the column or may bepositioned close enough to the inner surfaces to transfer heat withoutthe need to physically contact the column. Where physical contactbetween the heating device and inner surfaces of the column occurs, thefriction between the components is not so high as to apply a strain orundue stress on the column. If desired, the heating device may comprisea coating such as a non-stick coating or other slick coatings to reducefriction between the heating device and the inner coil surfaces. Inother instances, a thermally conductive track can be placed along and/orin contact with the inner surfaces of the coiled column, and the heatingdevice may ride along the track from an initial positon to anotherposition.

In some embodiments, the heating device may instead be configured tocontact an outer surface of the coiled column. For example, the heatingdevice can be configured as a U-shaped member (or C-shaped memberdepending on the orientation) that can be inserted along the length ofthe column from an inlet end of the column to an exit end of the column.One illustration is shown in FIG. 8. A heating device 810 comprises afirst end 812 and a second end 814. The heating device 810 is configuredto contact outer surfaces of a column 820 as the heating device is movedfrom an inlet end 822 of the column 820 toward an exit end 824 of thecolumn 820. Insulating member 830 is shown that surrounds the heatingdevice 810 and the column 820. If desired, an optional insulating member840 can be positioned in the inner space formed by the column 820 toreduce any thermal fluctuations caused by open space within the innerspace of the coiled column 820. While not shown, air can be blown intothe device from the end 824 of the column 820 toward the end 814 of theheating device 810 to provide a difference in temperature between theend 812 and the end 814 of the heating device 810. Alternatively, theheating device 810 may comprise individually controlled sections, e.g.,similar to the sections of FIG. 7, to permit temperature control ofvarious sections of the heating device 810. In use of the heating device810, the heating device 810 can be inserted toward the end 824 of thecolumn 820 in a similar manner as described in connection with FIGS.1A-6B. The exact speed at which the heating device 810 is inserted mayvary depending on the analyte components on the column 820. The heatingdevice 810 can be used to provide a substantially linear thermalgradient or can be used to provide a non-linear gradient. Once theheating device 810 is inserted to a desired final position, the heatingdevice 810 may be moved back to an initial position prior to asubsequent sample injection into the device. While the heating device810 is described as being moved, the column 820 could instead be movedwhile the heating device 810 remains stationary. Alternatively, both thecolumn 820 and the heating device 810 can be moved if desired.

In some instances, it may be desirable to thermally couple the heatingdevice to both the inner and outer surfaces of a coiled column such thatno temperature gradients exist or are minimized locally within thecolumn as a result of open space. One configuration is shown in FIG. 9.The heating device 910 comprises arms 912, 914 and an inner body 916.The heating device 910 is configured to contact outer surfaces of acolumn 920 using the arms 912, 914 and inner surfaces of the column 920using the body 916 as the heating device 910 is moved from an inlet end922 of the column 920 toward an exit end 924 of the column 920.Insulating member 930 is shown that surrounds the heating device 910 andthe column 920. While not shown, air can be blown into the device fromthe end 924 of the column 920 toward the end of the heating device 910to provide a difference in temperature between the ends of the heatingdevice 910. Alternatively, the heating device 910, e.g. the arms, thebody or both, may comprise individually controlled sections, e.g.,similar to the sections of FIG. 7, to permit temperature control ofvarious sections of the heating device 910. In use of the heating device910, the heating device 910 can be inserted toward the end 924 of thecolumn 920 in a similar manner as described in connection with FIGS.1A-6B. The exact speed at which the heating device 910 is inserted mayvary depending on the analyte components on the column 920. The heatingdevice 910 can be used to provide a substantially linear thermalgradient or can be used to provide a non-linear gradient. Once theheating device 910 is inserted to a desired final position, the heatingdevice 910 may be moved back to an initial position prior to asubsequent sample injection into the device. In some embodiments, thetemperature provided by the arms 912, 914 and 916 may be about the samefor a selected radial plane of the heating device 910. If desiredhowever, any one of the arms 912, 914 or body 916 may have a differenttemperature than other portions of the heating device 910. For example,the arms 912, 914 may be present at a first temperature and the body 916may be present at a second temperature different from the firsttemperature. If desired, the arms 912, 914 may be electrically coupledto a first power source and the body 916 may be electrically coupled toa second power source. In alternative configurations, the arms 912, 914and body 916 are electrically coupled to a common power source. Whilethe heating device 910 is described as being moved, the column 920 couldinstead be moved while the heating device 910 remains stationary.Alternatively, both the column 920 and the heating device 910 can bemoved if desired.

In certain embodiments, the columns used with the heating devicedescribed herein may not necessarily be coiled. For example, a cartridgecolumn, wafer column, etc. that is substantially planar may be used witha heating device. Referring to FIG. 10, a wafer column 1020 is shown asa substantially planar device. The column 1020 is thermally coupled to aheating device 1010 which can take the form of a plate. The columncomprises an inlet end 1022, which can be fluidically coupled to aninjector or other sample introduction device (not shown), and an outletend 1024, which can be fluidically coupled to a detector (not shown) orother device. The heating device 1010 comprises a first end 1012 and asecond end 1014. In use of the heating device 1010, the heating devicecan be moved from the end 1022 of the column 1020 to the end 1024 of thecolumn 1020. The heating device 1010 may physically contact the topsurface of the wafer column 1020 or may ride, for example, along agroove or track in the housing of the column 1020. The exact type ofgradient provided by the heating device 1010 may be linear,substantially linear or non-linear. While not shown, air can be providedfrom the end 1024 of the column toward the end 1014 of the heatingdevice 1010 to provide a thermal gradient from the end 1012 to the end1014. Once the end 1014 of the heating device 1010 is at or near the end1024 of the column 1020, the heating device 1010 can be moved back to aninitial position prior to injection of an additional sample into thewafer column 1020. Alternatively, the heating device 1010 may remainstationary and the column 1020 may instead be moved. If desired, boththe heating device 1010 and the column 1020 can be moved.

In some configurations where a wafer column is used, it may be desirableto configure the heating device with a plate that can provide thermalcoupling to each side of the wafer column. Referring to FIG. 11, a wafercolumn 1120 is shown as a substantially planar device. The column 1120is thermally coupled to a heating device that comprises a first plate1000 and a second plate 1010. The column 1120 comprises an inlet end1122, which can be fluidically coupled to an injector or other sampleintroduction device (not shown), and an outlet end 1124, which can befluidically coupled to a detector (not shown) or other device. The plate1100 comprises a first end 1102 and a second end 1104, and the plate1110 comprises first end 1112 and a second end 1114. In use of theheating device, the plates 1100, 1110 can be moved from the end 1122 ofthe column 1120 to the end 1124 of the column 1120. Each of the plates1100, 1110 may physically contact a surface of the wafer column 1120 ormay ride, for example, along a groove or track on the housing of thecolumn 1120. The exact type of gradient provided by the plates 1000,1110 may be linear, substantially linear or non-linear. While not shown,air can be provided from the end 1124 of the column toward the ends1104, 1114 to provide a thermal gradient between the ends of the plates1100, 1110. Once the ends 1004, 1114 of the plates are at or near theend 1124 of the column 1120, the plates 1100, 1110 can be moved back toan initial position prior to injection of an additional sample into thewafer column 1120. The plates 1100, 1110 typically are at the sametemperature for a selected radial plane, but if desired, the plates1100, 1110 may provide different temperatures or different thermalgradients to the column 1120. In an alternative configuration, theplates 1100, 1110 may remain stationary and the column 1120 may insteadbe moved. In other instances, both the plates 1100, 1110 and the column1120 can be moved.

In certain configurations, the body of the heating device may compriseholes or apertures in certain portions to provide a thermal gradientfrom one end of the heating device to the other. Referring to FIG. 12, aheating device 1200 comprises a body with a first end 1210 and a secondend 1220. The body may be generally solid at the end 1210 and maycomprise apertures 1230 and 1232 at the end 1220 to permit air to flowinto the end 1220 and cool the end 1220 to a temperature below the end1210. While the apertures 1230 and 1232 are shown as being positionedalong the longitudinal axis L of the heating device 1200, the aperturesmay instead be positioned radially in the end 1220 of the device 1200 topermit some air to flow into that end of the heating device 1200 in thelongitudinal direction of the axis L. Further, the apertures need not becircular but instead may take other shapes and sizes includingrectangular, square, elliptical, etc. In addition, different aperturesneed not have the same size or shape. In some instances, the heatingdevice may comprise removable plugs that can be inserted into theapertures to close up the space. For example, the plugs can be removedwhen a larger thermal gradient is desired or be present when a lowerthermal gradient is desired. The plugs may fit through a friction fit orthrough other means as desired. In some instances, the plugs maycomprise the same material as the rest of the body of the heating devicesuch that the thermal properties of the plug are about the same as therest of the body of the heating device. In certain examples, the heatingdevice may comprise a sliding cylinder portion that can be movedrelative to the other portion of the heating device to permit theapertures to be open or to be closed depending on the positon of thesliding cylinder. Alternatively, the cylinder portion can be rotated toeither open or close the apertures of the heating device to at leastsome degree as desired.

In certain configurations, the heating device may comprise a solid bodybut different portions of the heating device may comprise differentmaterials. For example, materials that provide a different temperaturefor a selected applied current may be present to tune the thermalgradient provided by the heating device even further. The differentmaterials can be brazed to each other, welded to each other, soldered toeach other or otherwise coupled to each other in some manner, e.g.,through a material such as a high temperature adhesive or ceramic. Ifdesired, more than two different materials can be present to provide forfurther temperature control of the heating device. In someconfigurations, a coating of a particular material may be present oncertain areas of the heating device and absent from other areas of theheating device to provide for further temperature control of the heatingdevice. In other instances, a coating may be present along the surfacesof the heating device, but the overall thickness of the coating may bedifferent in different areas to permit further control of thetemperature.

In certain examples, the heating device may be configured to functionwith an accessory device that remains coupled to the column. Forexample, a chromatography column can be coiled around a hollow cylindercomprising a central aperture that can receive the heating device. Thehollow cylinder can transfer heat from the heating device through thehollow cylinder and to the coiled column. The use of a hollow cylindermay be desirable, for example, where the column is delicate or wheredirect contact of the moving heating device to the column is notdesired. The accessory device need not be a hollow cylinder but mayinstead be a series of tracks coupled to the column, a cage, a barrel orother forms which can couple to a chromatography column and provide forthermal transfer from the heating device to the chromatography column.

In certain configurations, the heating devices described herein may beconfigured for use with non-coiled columns. For example, the heatingdevice may be configured as a cylinder or cartridge with a centralopening that can receive a non-coiled chromatography column. In someinstances, the central opening may be sized and arranged so that it isslightly larger than the dimensions of the non-coiled chromatographycolumn. In use, the heating device is moved so that the column entersinto the central opening of the heating device, and a thermal gradientis provided to that portion of the column positioned within the centralopening of the heating device. The heating device can be moved from theinlet of the column toward the outlet to provide a thermal gradientalong the length of the column. Alternatively, the column can be movedinto the heating device, or both the column and the heating device canbe moved.

In certain embodiments, the heating devices described herein may be usedwith a non-conductive column. For example, the columns may be consideredinsulators or otherwise are substantially electrically non-conductive.This configuration prevents transfer of the current applied to theheating device to the column itself when/if the heating device contactsthe column. Without wishing to be bound by any particular theory,charging of the column may induce local thermal gradients within thecolumn itself as those portions of the column can heat up, which can actto reduce the overall precision and accuracy of chromatographic runs.Where a conductive column housing is present, an insulating sleeve orother electrically non-conductive member may be coupled to the housingto prevent current transfer from the heating device to the column. Ifdesired, however, a conductive column can be used. For example, aconductive column can be separated from a heating device through one ormore insulator tracks designed for the heating device to ride along.

In certain configurations, the heating device described herein canfunction as, or similar to, a resistive heater or a thermoelectricheater. For example, a current can be applied to a conductive portion ofa resistive heating element in the heating device to provide the heat.In other instances, a solid state active heat pump, e.g., athermoelectric heater, may be present within the heating device andfunctions to provide a temperature difference based on current flowthrough the device. In some instances, the entire body of the heatingdevice may comprise or function as a resistive heating element or athermoelectric heater, whereas in other instances one or more resistiveheating elements of thermoelectric heaters can be present and used totransfer heat to other portions of the heating device. In operation, asubstantially constant current may be applied to the heating deviceduring the separation, and a thermal gradient may be provided by coolingone area of the heating device and/or permitting increased air flow toone area of the heating device. If desired, the applied current may bealtered during the chromatographic separation to alter the thermalgradient. Where the heating device is configured with one or moresections, different types or a different number of heating devices maybe present in different sections to provide temperature differences indifferent sections of the heating device.

In some instances, the heating device may be produced using conductiveor semi-conductive materials that can provide the desired heating and/ortemperature difference between different areas of the heating device.Suitable materials include but are not limited to, nickel, chromium andalloys and materials including nickel and chromium, e.g., Nichrom,tungsten, silicon or other materials or combinations of metals ormaterials. In some instances, the heating device has a thermal masssubstantially larger than the thermal mass of the column. Movement ofthe high thermal mass heating device adjacent to a portion of the lowthermal mass column (or movement of the column toward the heatingdevice) can cause the temperature of that portion of the column toquickly become about the same temperature as that area of the heatingdevice. The high thermal mass of the heating device can reduce thermalfluctuations and provide rapid temperature adjustment of different areasof the column. In some instances, the heating device has sufficientthermal mass such that the temperature of an adjacent column portionrises to about the same temperature as the heating device within lessthan about 1 second.

In certain configurations, the heating devices described herein may beused with more than a single column. For example, a single heatingdevice can be configured for use with two or more coiled columns, two ormore wafer columns or column bundles, e.g., capillary column bundles,etc. Two illustrations are shown in FIGS. 13 and 14. Referring to FIG.13, a heating device 1300 is shown that can include two members 1310,1320 and that can be moved into inner space formed by each of columns1330, 1340. An insulating member 1350 may be present between the twocolumns 1330, 1340 to prevent heat transfer away from each of thecolumns 1330, 1340 to any open space between the columns 1330, 1340.While the coils of each of the columns 1330, 1340 are shown as beingspaced closer together toward ends 1334, 1344, the spacing of the columncoils can be about the same from the end 1332 to the end 1334 and fromthe end 1342 to the end 1344. In use of the heating device 1300 and thecolumns 1330, 1340, the heating device 1300 can be inserted into theopen space 1331, 1341 formed by coiling of the columns 1330, 1340,respectively. The speed of insertion of the heating device 1300 can becontrolled by a motor (not shown) or other device coupled to the heatingdevice 1300. In the configuration shown in FIG. 13, the positions of themembers 1310, 1320 are fixed relative to each other and are generally inthe same plane with each other. If desired, however, one of the members1310, 1320 can be offset or can be individual controlled so movement ofthe members 1310, 1320 occurs at a different speed or rate. Similarly,the members 1310, 1320 need not be sized or configured the same as eachother and may take different lengths, include different materials orhave different shapes or overall construction. While not shown, coolingair can be introduced from the ends 1334, 1344 toward the ends 1332,1342 of the columns 1330, 1340 to assist the heating device 1300 inproviding a thermal gradient to the columns 1330, 1340. While theheating devices 1310, 1320 of FIG. 13 are described as being moved, thecolumns 1330, 1340 could instead be moved or both the heating devices1310, 1320 and the columns 1330, 1340 can be moved.

Referring now to FIG. 14, a heating device configured for use with twowafer columns is shown. The heating device 1410 is configured as asingle plate comprising a first end 1412 and a second end 1414. Twowafer columns 1420 and 1430 are shown positioned adjacent to the end1414 of the heating device 1410. Optional insulating members 1440, 1450can be present to reduce heat transfer away from the wafer columns 1420,1430. In use of the heating device 1410, the heating device 1410 can beinserted into the space 1435 between the wafer columns 1420, 1430 untilit reaches an exit end 1424, 1434 of the wafer columns 1420, 1430 toprovide a thermal gradient to different portions of the wafer columns1420, 1430 during movement of the heating device 1410. While not shown,cooling air can be introduced from the ends 1424, 1434 toward theheating device 1410 to assist the heating device 1410 in providing athermal gradient to the wafer columns 1420, 1430. While a heating deviceconfigured as a single plate 1410 is shown in FIG. 14, where two or morewafer columns are present, the heating device may comprise more than asingle plate or be configured as a heating device that takes forms otherthan a plate. In addition, the columns 1420, 1430 may be moved insteadof moving the heating device 1410, or both the heating device 1410 andthe columns 1420, 1430 can be moved.

In certain embodiments, a column bundle which comprises a plurality ofcoiled column may be used with a heating device. The heating device canbe configured to contact or be adjacent to inner surfaces of columncoils or outer surfaces of column coils or combinations thereof topermit thermal transfer from the heating device to each column in thebundle. In some instances, column bundles can be arranged inantiparallel configurations such that movement of the heating device inone direction thermally couples the heating device to a first column,and movement of the heating device in an anti-parallel directionthermally couples the heating device to a second column. Oneillustration is shown in FIG. 15. A heating device 1510 is shownpositioned between a first column 1520 and a second column 1530. Whenthe heating device 1510 is moved in the direction of arrow 1542 into theinner space 1541 formed from the coiled column 1520, the heating device1510 provides a thermal gradient along the column 1520. If desired, air,for example, can be blown from the end of the column 1520 toward theheating device 1510 to assist the heating device 1510 in providing thethermal gradient to the column 1520. The heating device 1510 may then bemoved antiparallel in the direction of arrow 1544 to thermally couplethe heating device 1510 to the column 1530. If desired, air can be blownfrom the end of the column 1530 toward the heating device 1510 to assistthe heating device 1510 in providing the thermal gradient to the column1530. The heating device can then be moved back to its initial orstarting position. In some instances, the two columns 1520, 1530 caneach receive the same sample from an introduction device, whereas inother examples the columns 1520, 1530 may receive different samples,e.g., from different injectors or sample introduction devices. While theheating device 1510 is shown as being about the same length as each ofthe spaces 1531, 1541, the heating device 1510 can be sized differently,e.g., can be T-shaped such that the upper portion of the T-shapedheating device can be thermally coupled to one or more columns bymovement.

In certain configurations, movement of the various heating devices andcolumns described herein can be provided using a motor coupled to theheating device or to the column or both. Where both the column and theheating device is moved, a common motor or two separate motors may bepresent. Numerous different types of motors are possible including, butnot limited to, electric powered motors, pneumatic motors, hydraulicmotors and other suitable motors. In some instances, the motor may be aDC electric motor such as a stepper motor that can be coupled to theheating device (or the column) through a shaft or other means. Theelectric motor can be used to adjust the position of the heating devicerelative to the column or the position of the column relative to theheating device. For example, the motor may be incrementally stepped toinsert the heating device further into the space formed by coiling ofthe column. Referring to FIG. 16A, a motor 1620 coupled to a heatingdevice 1630 through a shaft 1625 is shown. The heating device 1630 isshown as being configured to insert into open space 1642 formed from acoiled column 1640. In use of the motor 1620, the motor 1620 can beconfigured to move the heating device 1630 into the space 1642 at adesired or controlled rate. For example, the rate at which the motor1620 moves the heating device 1630 can control the temperature along thelength of the column 1640. While not shown, a fan or other device canprovide air from the end of the column 1640 to assist in providing athermal gradient from the heating device 1630. Alternatively, a heatsink, cooling rod or Peltier cooler can be coupled to one end of theheating device 1630 to provide a lower temperature at that end. Ifdesired, the motor 1620 could instead be coupled to the column 1640 topermit movement of the column 1640 toward the heating device 1630.Alternatively, a second motor may be present and coupled to the column1640 to permit movement of both the column 1640 and the heating device1630.

In certain configurations, the speed of the movement may be controlled,for example, by a processor electrically coupled to the motor. Asimplified block diagram is shown in FIG. 16B. A processor 1650 iselectrically coupled to a motor 1620 which is coupled to a heatingdevice 1630, through a shaft 1625, that is shown partially inserted intoa space 1642 formed by coiling a column 1640. The processor 1610 cancontrol the motor 1620 and in turn control the speed at which theheating device 1630 is inserted into the space 1642. While not shown,the processor 1610 can also be electrically coupled to a power source,which itself can be coupled to the heating device 1630. The processor1610 may control the level of current provided from the power source tothe heating device 1630 to control the temperature of the heating device1630. Where a fan (not shown) is present to assist in providing thethermal gradient from the heating device 1630 to the column 1640, thefan can be electrically coupled to the processor 1610 to control the fanspeed. The processor 1610 can also be used to control thechromatographic separation if desired and/or may be used by the detector(not shown) and/or a display to output the results of a chromatographicrun.

In certain configurations, the motor 1620 may be a brushed or brushlesselectric motor configured to incrementally move the heating device 1630into the space 1642. If desired, one or more gears, transmissions, etc.can be present to further assist in movement of the heating device 1630in a desired direction and at a desired speed. Similar devices can beused where a motor is coupled to a column. If desired, more than asingle electric motor can be present to further control movement of theheating device (or column) in a desired direction and/or at a desiredspeed. In some instances, the exact configuration of the electric motormay depend on the particular power source used. As noted herein, DCpower sources may be desirable in portable applications where thechromatography systems are used remotely from an available AC powersource. AC power sources can also be used in addition to, or in place,of, a DC power source. Illustrative motors that can be used include, butare not limited to, a brushed DC motor, a brushless DC motor, an ACinduction motor, a permanent magnet synchronous motor, a stepper motorand a switched reluctance motor. The motor may be a single phase motoror a multi-phase motor and can be controlled in many different mannersincluding voltage modulation such as, for example, pulse widthmodulation or other means.

In other configurations, the motor 1620 can be configured as a pneumaticmotor that can use air pressure to move the shaft 1625 and the heatingdevice 1630 in a desired direction. The pneumatic motor may comprise acompressor or use air pressure from the chromatography system to convertthe air pressure into mechanical movement of the heating device 1630.Where a pneumatic motor is used, the pneumatic motor may comprise, or beconfigured as, a diaphragm or a piston actuator that can move theheating device 1630 into and out of the space 1642. For example, thepneumatic motor can be configured as a spring piston coupled to asolenoid valve which can be actuated open by the processor 1610 to applypressure to the piston and force the piston in a certain direction andcause movement of the heating device 1630. The valve can be closed andthe pressure released to permit the spring to return to its restingposition and retract the piston and heating device 1630. Pneumaticmotors may be desirable to use, for example, in volatile environmentswhere sparks should be avoided or where available DC or AC power islimited. If desired, a pneumatic motor can instead be coupled to acolumn to control movement of the column.

In certain embodiments, the motor may be configured as a hydraulic motorthat can be coupled to the heating device 1630, for example, through oneof more gears to permit movement of the heating device 1630 in a desiredmanner. While not wishing to be bound by any particular theory, thehydraulic motor may convert hydraulic pressure into mechanical movement.The exact configuration of the hydraulic motor may vary and may include,but is not limited to, gear and vane hydraulic motors, gerotor hydraulicmotors, axial plunger hydraulic motors, and radial piston hydraulicmotors. Where the hydraulic motor is designed to cause angular rotation,one or more gears, differentials or transmissions may be present toconvert the angular motion to a linear motion to permit movement of theheating device 1630 in a desired direction. In some instances, it may bedesirable to use a first hydraulic motor to move the heating device in afirst direction and use a second hydraulic motor to move the heatingdevice back to an initial position. The braking effect provided when thehydraulic motor is not in use can be used to assist in control of themovement of the heating device at a desired speed. If desired, ahydraulic motor can instead be coupled to a column to control movementof the column.

While various embodiments described herein refer to the heating devicebeing moved relative to a stationary column, if desired, the heatingdevice may instead remain stationary and the column can be moved. Forexample, the column can be coupled to one or more of the motorsdescribed herein and moved into the heating device (or alongside of theheating device) at a desired speed to provide a thermal gradient to themoving column from the stationary heating device. Further, if desiredboth the heating device and the column can be moved to further controlthe application of the thermal gradient to the column. For example, itmay be desirable to use motors having a different number of steps withthe heating device and with the column to provide for finer movement ofthe heating device and the column. A single processor or multipleprocessors can be used to control the two motors where both the heatingdevice and the column are moved. If desired, a single motor can be usedto control movement of both the heating device and the column.

In certain embodiments, the exact power source used with the heatingdevice may depend on the particular configuration of the chromatographysystem. Where AC power is used, the AC power may be provided from autility grid, a generator or other AC power sources. Where thechromatography system takes the form of a portable device or system (asdescribed in more detail below), the power source may be a DC powersource including, but not limited to, solar cells, batteries or otherelectrochemical cells, fuel cells, wind turbine generators, crankgenerators, or DC power provided from a remote power source such asdiesel generator, tractor power take off coupled to an alternator orgenerator, or other fuel powered engines that can generate DC power. Thepower source may be configured as a hot swappable power source so as thepower source is drained an additional power source can be interchangedwith it. In some instances, the systems may include two or moredifferent power sources to permit swapping of the power sources withoutneeding to shut down the entire system. For example, the system may bedesigned to use a first power source until the first power source drainsto a certain level and then automatically switch over to a second powersource. The first power source may then be swapped out with a chargedpower source. Once the second power source drains to a certain level,the system can revert to using the charged first power source to permitthe user to swap out the drained second power source. Alternatively, thesystem can include back up power that is used when a primary powersource is absent or too low for operation. The primary and/or back uppower sources can be on board the device or can be coupled to the deviceusing a wired interconnect or wireless coupling such as through the useof a wireless connection, e.g., an inductive charger or inductive powersource.

In certain embodiments, the exact temperature gradient provided from theheating device may vary depending on the species to be analyzed. In someinstances, one end of the heating device may provide a temperature of upto about 350 degrees Celsius or up to about 400 degrees Celsius or up toabout 450 degrees Celsius or even up to about 500 degrees Celsius.Higher temperatures are also possible if desired. The temperature maydecrease linearly at a rate of about 1 degrees Celsius/cm to about 50degrees Celsius/cm or even greater than 50 degrees Celsius/cm along thelength of the heating device from the hot end to the colder end. Inother instances, the temperature may decrease in a non-linear fashionwith curves, stepped temperatures or plateaus present over a desireddistance, e.g., a thermal gradient having a profile that issubstantially monotonically non-increasing and has a positive secondderivative as described in U.S. Ser. No. 14/488,013 filed on Sep. 16,2014 can be implemented using the heating device. If desired, one ormore temperature sensors, e.g., thermocouples or other temperaturemeasuring devices, can be present at desired areas of the heating deviceto provide a measure of the temperature at that area. In someconfigurations, the temperature sensors can be electrically coupled tothe processor to permit the processor to control the current levelprovided to the heating device and alter the temperature as desired.

In certain embodiments, the heating device described herein may bepresent in a gas chromatography (GC) system. Referring to FIG. 17, atypical GC system 1700 comprises an injector 1710 fluidically coupled toa column 1720 through a fluid line 1712. The injector is fluidicallycoupled to a pressure source 1705 that can provide a gas, e.g., acarrier gas, to the injector 1710 through a fluid line 707 and/or to thecolumn 1730 through the fluid line 1712. A heating device 1730 is shownthat can be inserted into the open space formed by the column 1720 toprovide a thermal gradient to the column 1720. A fan (not shown) can bepositioned on the other side of the column 1720 to blow air into theopen space formed by the column 1720. The heating device 1730 and column1720 can be positioned in an oven 1735 configured to maintain the column1720 at a desired temperature before the heating device 1730 is insertedinto the space formed from the coiled column 1720. The injector 1710 maybe, for example, a split or splitless injector, or other types ofinjectors commonly used in GC devices. The column 1720 can befluidically coupled to a detector 1740 through a fluid line 1723. Asnoted herein, the column 1720 may take many forms including capillarycolumns, capillary column bundles, wafer columns, column cartridges orother types of columns. The heating device 1730 may take many forms asdescribed herein. The detector 1740 may also take many forms dependingon the type of analyte to be detected. For example, the detector 1740may be a mass spectrometer, a thermal conductivity detector, a flameionization detector, a flame photometric detector, a photoionizationdetector, an infrared detector, a catalytic combustion detector, adischarge ionization detector, an electron capture detector, athermionic detector, a nitrogen-phosphorous detector or other detectorcommonly used with GC devices.

In certain configurations, the GC devices comprising a heating devicemay be hyphenated with one or more other devices. For example, tandemGC-GC device, GC-mass spectrometer (MS) devices or other tandem deviceswhere one of the device comprises a heating device as described hereinmay be used. In certain embodiments, the GC devices described herein maybe used with a mass spectrometer. A block diagram of one system is shownin FIG. 18. The GC-MS device 1800 comprises a sample introduction device1810, a GC device 1820 comprising a heating device, a mass analyzer1830, a detector 1840, a processing device 1850 and an optional display(not shown). The sample introduction device 1810, the GC device 1820,the mass analyzer 1830 and the detector 1840 may be operated at reducedpressures using one or more vacuum pumps such as roughing pumps and/orturbomolecular pumps. In certain examples, however, only the massanalyzer 1830 and the detector 1840 may be operated at reducedpressures. The sample introduction device 1810 may include an inletsystem configured to provide sample to the GC device 1820. The inletsystem may include one or more batch inlets, direct probe inlets and/orchromatographic inlets and/or injectors. For example, the sampleintroduction device 1810 may be an injector, a nebulizer or othersuitable devices that may deliver solid, liquid or gaseous samples tothe GC device 1820. The GC device 1820 typically comprises a column andheating device as noted in connection with FIG. 17. The mass analyzer1830 may take numerous forms depending generally on the sample nature,desired resolution, etc. and exemplary mass analyzers are discussedfurther below. The detector 1840 may be any suitable detection devicethat may be used with existing mass spectrometers, e.g., electronmultipliers, Faraday cups, coated photographic plates, scintillationdetectors, etc., and other suitable devices that will be selected by theperson of ordinary skill in the art, given the benefit of thisdisclosure. The processing device 1850 typically includes amicroprocessor and/or computer and suitable software for analysis ofsamples introduced into GC-MS device 1800. One or more databases may beaccessed by the processing device 1850 for determination of the chemicalidentity of species introduced into MS device 1800. Other suitableadditional devices known in the art may also be used with the MS device1800 including, but not limited to, autosamplers, such as AS-90plus andAS-93plus autosamplers commercially available from PerkinElmer HealthSciences, Inc.

In certain embodiments, the mass analyzer of MS device 1800 may takenumerous forms depending on the desired resolution and the nature of theintroduced sample. In certain examples, the mass analyzer can be ascanning mass analyzer, a magnetic sector analyzer (e.g., for use insingle and double-focusing MS devices), a quadrupole mass analyzer, anion trap analyzer (e.g., cyclotrons, quadrupole ions traps, aminiaturized toroidal ion trap), time-of-flight analyzers (e.g.,matrix-assisted laser desorbed ionization time of flight analyzers), andother suitable mass analyzers that may separate species with differentmass-to-charge ratios. The GC devices disclosed herein may be used withany one or more of the mass analyzers listed above and other suitablemass analyzers.

In certain other examples, the chromatography devices disclosed here maybe used with existing ionization methods used in mass spectroscopy. Forexample, electron impact sources in combination with a GC device may beused. In other examples, chemical ionization sources in combination witha GC device may be used. In yet other examples, field ionization sourcesin combination with a GC device may be provided. In still otherexamples, the GC devices may be used with desorption sources such as,for example, those sources configured for fast atom bombardment, fielddesorption, laser desorption, plasma desorption, thermal desorption,electrohydrodynamic ionization/desorption, etc. In yet other examples,the GC devices may be configured for use with thermospray ionizationsources, electrospray ionization sources or other ionization sources anddevices commonly used in mass spectroscopy. It will be within theability of the person of ordinary skill in the art, given the benefit ofthis disclosure, to design suitable devices for ionization that also usea chromatography device or GC device as described herein.

In certain examples, the chromatography devices described herein can beused in portable devices. For example, a small portable gas cylinder orgas cartridge similar to the size of a small propane tank, e.g., a 1liter tank, a 1-gallon tank or a 5-gallon tank, can be filled withhelium or other suitable carrier gas or air can be used as a carriergas. The gas cylinder can be fluidically coupled to the GC device topermit chromatographic separations and/or analyses. Illustrativeportable device applications include, but are not limited to, soilanalysis, hydrocarbon fluid analysis, air space analysis or otherenvironment analysis, pharmaceutical analysis, e.g., ADME analyses, foodscience analysis, perfume analysis, essential oil analysis, veterinaryassays or other chemical tests commonly performed in non-laboratorysettings.

In some examples, the GC device may be present on a mobile vehicle suchas a military vehicle, an aircraft, a satellite, a spaceship or shuttle,an unmanned aerial vehicle, a rocket or missile or other vehiclescommonly used in the military and/or in aerospace settings. The vehiclecan be propelled, for example, using an engine or through combustion orburning of liquid or gas fuels or may be powered using an electricengine, hybrid engine or a fuel cell. Alternatively, the GC device maybe present on devices commonly used by the national oceanic andatmospheric administration including, for example, weather balloons,hurricane aircraft, portable weather devices, ocean buoys or otherdevices which can transmit information about the atmosphere.

In yet other configurations, the GC device may be configured similar toa briefcase that can be transported by a human. Referring to FIG. 19, anillustration of a portable GC device is shown. The GC device 1900comprises a housing 1905 comprising a handle 1910 to assist in carryingof the device 1900. If desired, a carrying strap, wheels or otherdevices may be present in addition to the handle 1910 or in place of thehandle 1910. While the exact dimensions of the device 1900 may vary,illustrative dimensions are about 25-35 cm wide by about 35-45 cm longby about 15-25 cm thick. The exact weight of the device may varydepending on the components present, but illustrative weights include,but are not limited to, about 7.5 kg to about 30 kg, more particularlyabout 8 kg to about 20 kg or about 10 kg to about 15 kg. While notshown, a cover may be present to prevent damage to a display screen orother device present on a top surface of the housing 1905. Where thedevice is designed to provide GC-MS analyses, the device 1900 maycomprise one or more turbo molecular pumps and/or one or more roughingpumps. The exact type of power source present in the device 1900 mayvary from batteries, fuel cells, solar cells or other power sourcesdescribed herein. In some instances, the power source may be selected topermit about 20-50 analyses until the power source needs to be replacedor recharged. A carrier gas can be coupled to the device 1900 or thedevice 1900 may comprise an onboard carrier gas or carrier gas cylinderor carrier gas cartridge (which can be replaced or recharged asdesired). In some instances, enough carrier gas may be present to permitabout 130-170 analyses prior to needing to replace or recharge thecarrier gas. The exact carrier gas used can vary and illustrativecarrier gases include, but are not limited to, hydrogen, helium, air,nitrogen, argon or other gases. The device 1900 may comprise a sampleintroduction device such as an injector to permit a user to inject asample using a syringe. Both liquid and gas samples can be injected asdesired. The device 1900 may comprise a processor, a keypad or othercomponents to facilitate use of the device 1900. In some instances, thedevice 1900 may be configured with a miniaturized toroidal ion trap orother MS devices. The device 1900 may further include a chromatographycolumn, an oven and a detector as noted in connection with FIG. 17.

In some instances, it may be desirable to use two separate heatingdevices. For example, the heating devices can be held at differenttemperatures so there is a thermal gradient between the two heatingdevices. In some instances, one heating device is thermally coupled to acolumn and then moved away from the column as the second heating devicebecomes thermally coupled to the column and is moved toward the column.In some examples, the first heating device can be thermally coupled to acolumn to permit equilibration of the column at substantially the sametemperature as the first heating device. The first heating device maythen be translated so it is moved completely away from the column priorto insertion of the second heating device into the column. The secondheating device can then be inserted into the column to provide a thermalgradient as the second heating device is inserted.

Referring to FIG. 20, one illustration of a system comprising twoheating devices is shown. The system comprises a column 2010 woundaround a substrate 2020. The substrate 2020, e.g., a glass tube or otherdevice, is sized and arranged to receive each of heating devices 2030,2040. One of the heating devices 2030, 2040 can be cooled to provide afirst temperature, and the other of the heating devices 2030, 2040 canbe heated to provide a second temperature higher than the firsttemperature. Each of the heating devices 2030, 2040 can be configuredsimilar to any of the other heating devices described herein and may beconfigured to surround an outer surface of the column 2010 or both aninner surface of the column 2010 and outer surface of the column 2010.In use of the two heating devices 2030, the heating device at the lowertemperature can first be thermally coupled to the column 2010 as shownin FIG. 21A. The device 2030 can then be removed from the interiorsurface of the column 2010, and the device 2040 can be insertedlongitudinally into the column 2010 to provide a thermal gradient. Theexact temperature differential of the heating devices 2030, 2040 mayvary and illustration temperature differences are about 150 deg.Celsius, 200 deg. Celsius, 250 deg. Celsius or more. In some examples,the heating device 2030 can be held at about 50-60 deg. Celsius, and theheating device 2040 can be held at about 290-300 deg. Celsius. One ormore of the heating devices 2030, 2040 may be thermally coupled to a fanto permit alteration of the temperature of that particular heatingdevice. The insertion/removal rates of the heating devices 2030, 2040can also vary. For example, the heating devices can be inserted at arate of about ⅛-½ inch every 10 seconds into the interior of the column2010. If desired, for example, insertion of the heating devices can becontrolled in a smooth manner using a stepper motor or other device.

In certain instances, the heating devices 2030, 2040 can be mechanicallycoupled to each other so when one of the heating devices 2030, 2040 isinserted into the column 2010, the other one of the heating devices2030, 2040 moves in generally the same longitudinal direction. Thelongitudinal spacing between the heating devices 2030, 2040 can beselected so that the heating devices 2030, 2040 are not inserted intothe interior of the column 2010 at the same time. The heating devices2030, 2040 can be sized and arranged so they do not contact the interiorsurface of the column 2010 but are close enough to transfer heat to thecolumn 2010. To enhance thermal transfer, the glass tube 2020 (FIG. 20)can be omitted entirely if desired so heat is transferred directly tothe column 2010.

In some embodiments, the heating devices 2030, 2040 can be configured asrods, plates, segmented rods, separate rods, bundled rods or configuredin other manners. In some instances, the heating devices rods maycomprise one or more apertures or bores which may optionally comprise aninternal fan to assist in heat transfer from the heating devices 2030,2040 to the column 2010. The exact material used in the heating devices2030, 2040 can vary and illustrative materials include metals such asaluminum or other solid materials which can retain heat well.

In some examples, the substrate around which the column is wound mayitself act as a heating device. For example and referring to FIG. 22, asystem is shown that comprises a column 2210 wound around a firstheating device 2220. A second heating device 2230 can be insertedlongitudinally into and out of the first heating device 2220. In use ofthe system of FIG. 22, the heating device 2220 is held at a firsttemperature to permit the column 2010 to equilibrate at thattemperature. The second heating device 2230 can then be inserted intothe interior of the first heating device 2230 to transfer heat to thefirst heating device 2220 and to the column 2210. Insertion of thesecond heating device 2230 into the first heating device 2220 canprovide a thermal gradient which can be used during a chromatographicseparation. If desired, the heating device 2230 can be configured tothermally transfer heat to outer surfaces of the column 2210 by slidingover the column 2210 and heating device 2220 rather than being insertedinto an interior area of the heating device 2220. While not shown, athird heating device can also be used with the column 2210 and theheating devices 2220, 2230.

In certain embodiments, the GC devices described herein can be used witha device or system comprising a computer or other device that includes aprocessor. The computer system can be separate or integral to the GCdevices. The processor can be used, for example, to control movement ofthe heating device, movement of the column, oven temperature, controlany fans or pumps, control a detector or to otherwise permit use of theGC devices in analyses. The computer system typically includes at leastone processor electrically coupled to one or more memory units toreceive input data from the GC device. The computer system may be, forexample, a general-purpose computer such as those based on Unix, IntelPENTIUM-type processor, Motorola PowerPC, Sun UltraSPARC,Hewlett-Packard PA-RISC processors, or any other type of processor. Oneor more of any type computer system may be used according to variousembodiments of the technology. Further, the system may be connected to asingle computer or may be distributed among a plurality of computersattached by a communications network. A general-purpose computer systemmay be configured, for example, to perform any of the describedfunctions including but not limited to: heating device movement control,temperature control gas flow rate control, detector monitoring, etc. Itshould be appreciated that other functions, including networkcommunication, can be performed and the technology is not limited tohaving any particular function or set of functions. Various aspects ofthe systems and methods may be implemented as specialized softwareexecuting in a general-purpose computer system. For example, a protocolconfigured to control and/or move the heating device to variouspositions can be implemented. The computer system may include aprocessor connected to one or more memory devices, such as a disk drive,memory, or other device for storing data. Memory is typically used forstoring programs and data during operation of the computer system.Components of the computer system may be coupled by an interconnectiondevice, which may include one or more buses (e.g., between componentsthat are integrated within a same machine) and/or a network (e.g.,between components that reside on separate discrete machines). Theinterconnection device provides for communications (e.g., signals, data,instructions) to be exchanged between components of the system. Thecomputer system typically is electrically coupled to a power source andone or more of the heating device, the column, the detector, the motor,etc. such that electrical signals may be provided to and from thecomputer and the electrically coupled devices. The computer system mayalso include one or more input devices, for example, a keyboard, mouse,trackball, microphone, touch screen, manual switch (e.g., overrideswitch) and one or more output devices, for example, a printing device,display screen, speaker. In addition, the computer system may containone or more interfaces that connect the computer system to acommunication network (in addition or as an alternative to theinterconnection device). The computer system may also include suitablecircuitry to convert signals received from the detector and/or othercomponents of the system. Such circuitry can be present on a printedcircuit board or may be present on a separate board or device that iselectrically coupled to the printed circuit board through a suitableinterface, e.g., a serial ATA interface, ISA interface, PCI interface orthe like or through one or more wireless interfaces, e.g., Bluetooth,WiFi, Near Field Communication or other wireless protocols and/orinterfaces.

In some instances where portable devices are used, the portable devicecan be designed to send remote signals to a central office or computersystem remote from the portable device. For example, informationrepresentative of analyses can be sent remotely to simplify the overallconstruction of the portable device and/or reduce power requirements ofthe portable device. Once the information is received remotely, it canbe used in determining the components and/or their amounts that wereanalyzed.

In other instances, the GC device can be configured to function with anaccessory device such as a mobile phone, a tablet, a laptop or otherdevice comprising an operating system that is separate from the GCdevice. In portable applications, it may be desirable to separate themobile device from the portable device to reduce overall powerconsumption by the portable device. Signals from the portable device maybe sent to the mobile device in a wired or wireless manner to permitviewing of the data/results on the mobile device. In some instances, themobile device can be coupled to the portable device through a dock or awired coupler, whereas in other instances, wireless communicationbetween the mobile device and the GC device may be used to transferinformation from the portable device to the mobile device. In addition,the mobile device can be pre-programmed or pre-configured to implementcertain operations that can automatically load from the mobile deviceinto the GC system. If desired, the mobile device can be designed foruse with two or more different systems to permit a single mobile deviceto implement the same or different operations on the two or moresystems. The mobile device may be pre-loaded with the GC routines,standard curves, user instructions, troubleshooting instructions orother information to assist in use of the GC devices. One or more menuscan be present on the mobile device to permit the user to select theparticular methodology of using the GC device or to troubleshootoperation of the GC device.

In certain embodiments, the storage system of the computer typicallyincludes a computer readable and writeable nonvolatile recording mediumin which GC routines can be stored that can be used by a program to beexecuted by the processor or information stored on or in the medium tobe processed by the program. The medium may, for example, be a disk,solid state drive or flash memory. Typically, in operation, theprocessor causes data to be read from the nonvolatile recording mediuminto another memory that allows for faster access to the information bythe processor than does the medium. This memory is typically a volatile,random access memory such as a dynamic random access memory (DRAM) orstatic memory (SRAM). It may be located in the storage system or in thememory system. The processor generally manipulates the data within theintegrated circuit memory and then copies the data to the medium afterprocessing is completed. For example, the processor may receive signalsfrom the detector and can display those signals in a format useful tothe end user. A variety of mechanisms are known for managing datamovement between the medium and the integrated circuit memory elementand the technology is not limited thereto. The technology is also notlimited to a particular memory system or storage system. In certainembodiments, the computer system may also include specially-programmed,special-purpose hardware, for example, an application-specificintegrated circuit (ASIC) or a field programmable gate array (FPGA).Aspects of the technology may be implemented in software, hardware orfirmware, or any combination thereof. Further, such methods, acts,systems, system elements and components thereof may be implemented aspart of the computer system described above or as an independentcomponent. Although a computer system is described by way of example asone type of computer system upon which various aspects of the technologymay be practiced, it should be appreciated that aspects are not limitedto being implemented on the described computer system. Various aspectsmay be practiced on one or more computers having a differentarchitecture or components. The computer system may be a general-purposecomputer system that is programmable using a high-level computerprogramming language. The computer system may be also implemented usingspecially programmed, special purpose hardware. In the computer system,the processor is typically a commercially available processor such asthe well-known Pentium class processor available from the IntelCorporation. Many other processors are available. Such a processorusually executes an operating system which may be, for example, theWindows 95, Windows 98, Windows NT, Windows 2000 (Windows ME), WindowsXP, Windows Vista, Windows 7, Windows 8 or Windows 10 operating systemsavailable from the Microsoft Corporation, MAC OS X, e.g., Snow Leopard,Lion, Mountain Lion or other versions available from Apple, the Solarisoperating system available from Sun Microsystems, or UNIX or Linuxoperating systems available from various sources. Many other operatingsystems may be used, and in certain embodiments a simple set of commandsor instructions may function as the operating system.

In certain examples, the processor and operating system may togetherdefine a computer platform for which application programs in high-levelprogramming languages may be written. It should be understood that thetechnology is not limited to a particular computer system platform,processor, operating system, or network. Also, it should be apparent tothose skilled in the art, given the benefit of this disclosure, that thepresent technology is not limited to a specific programming language orcomputer system. Further, it should be appreciated that otherappropriate programming languages and other appropriate computer systemscould also be used. In certain examples, the hardware or software can beconfigured to implement cognitive architecture, neural networks or othersuitable implementations. If desired, one or more portions of thecomputer system may be distributed across one or more computer systemscoupled to a communications network. These computer systems also may begeneral-purpose computer systems. For example, various aspects may bedistributed among one or more computer systems configured to provide aservice (e.g., servers) to one or more client computers, or to performan overall task as part of a distributed system. For example, variousaspects may be performed on a client-server or multi-tier system thatincludes components distributed among one or more server systems thatperform various functions according to various embodiments. Thesecomponents may be executable, intermediate (e.g., IL) or interpreted(e.g., Java) code which communicate over a communication network (e.g.,the Internet) using a communication protocol (e.g., TCP/IP). It shouldalso be appreciated that the technology is not limited to executing onany particular system or group of systems. Also, it should beappreciated that the technology is not limited to any particulardistributed architecture, network, or communication protocol.

In some instances, various embodiments may be programmed using anobject-oriented programming language, such as SmallTalk, Basic, Java,C++, Ada, or C# (C-Sharp). Other object-oriented programming languagesmay also be used. Alternatively, functional, scripting, and/or logicalprogramming languages may be used. Various configurations may beimplemented in a non-programmed environment (e.g., documents created inHTML, XML or other format that, when viewed in a window of a browserprogram, render aspects of a graphical-user interface (GUI) or performother functions). Certain configurations may be implemented asprogrammed or non-programmed elements, or any combination thereof.

In using systems comprising a processor, the processor can be designedto provide the various control and functions in a processing time, whichis typically orders of magnitude faster than operations that could beperformed manually. For example, the processor can be configured toimplement various functions in less than 1 millisecond, less than 100microseconds or even less than 10 microseconds to facilitate rapidcontrol of the GC devices. In some instances, signals provided from thedetector to the processor can be displayed or otherwise provided in nearreal-time to permit a user to view analyses quickly and without the needto manipulate the information manually. The processor can be configuredto simultaneously control multiple functions of the GC devicesincluding, for example, motor control, detector operation, gas flows,data display and other operations performed by the GC devices.

In certain embodiments, a kit comprising a heating device configured tothermally couple to a chromatography column at a first position, andinstructions for using the heating device with the chromatography columnto provide a thermal gradient to the chromatography column during achromatographic separation by moving the heating device in alongitudinal direction along the chromatography column from the firstposition to a second position different from the first position can beprovided. In some instances, the kit may also include a motor configuredto couple to the heating device to move the heating device in thelongitudinal direction, e.g., a stepper motor. The kit may be used, forexample, to retrofit existing GC devices with the moveable heatingdevices described herein. The kit may also comprise a processorconfigured to electrically couple to the motor and control movement ofthe heating device in the longitudinal direction. If desired, theexisting processor of a GC device can be replaced with the processor ofthe kit or the existing processor can be reprogrammed for use with theheating device. The kit may also include one or more chromatographycolumns including, but not limited to, a coiled capillary column, acoiled capillary column bundle, a wafer column and a non-coiledcapillary column. If desired a DC power source, e.g., at least one of anelectrochemical cell, a fuel cell, a solar cell or a wind turbineconfigured to provide a direct current, can be included in the kit.Additional DC power sources can also be included if desired.

In other configurations, a kit may comprise a heating device configuredto thermally couple to a chromatography column at a first position, andinstructions for using the heating device with the chromatography columnto provide a thermal gradient to the chromatography column during achromatographic separation by moving the chromatography column in alongitudinal direction along the heating device from the first positionto a second position different from the first position. In someconfigurations, the kit may include a motor, e.g., a stepper motor,configured to couple to the chromatography column to move thechromatography column in the longitudinal direction. The kit may beused, for example, to retrofit existing GC devices with the moveablecolumns described herein. The kit may also comprise a processorconfigured to electrically couple to the motor and control movement ofthe column in the longitudinal direction. If desired, the existingprocessor of a GC device can be replaced with the processor of the kitor the existing processor can be reprogrammed for use with the column.The exact nature of the moveable column of the kit can vary andillustrative columns include, but are not limited to, a coiled capillarycolumn, a coiled capillary column bundle, a wafer column and anon-coiled capillary column. If desired a DC power source, e.g., atleast one of an electrochemical cell, a fuel cell, a solar cell or awind turbine configured to provide a direct current, can be included inthe kit. Additional DC power sources can also be included if desired.

In certain embodiments, a method comprising providing a heating deviceconfigured to thermally couple to a chromatography column at an inletsection of the chromatography column in a first position and tothermally couple to the chromatography column at a section downstreamfrom the inlet section in a second position, the heating deviceconfigured to move in a longitudinal dimension along the chromatographycolumn from the first position to the second position during achromatographic separation to provide a thermal gradient during thechromatographic separation is provided. In certain instances, the methodmay comprise providing a substantially linear thermal gradient duringthe chromatographic separation by maintaining the heating device at asubstantially constant temperature during the chromatographicseparation. In other instances, the method may comprise providing asubstantially linear thermal gradient during the chromatographicseparation using a cooling device thermally coupled to the heatingdevice. In certain configurations, the cooling device can be movedduring the chromatographic separation. In other configurations, thecooling device may remain in a stationary position during thechromatographic separation. In some examples, the method comprisesmoving the heating device from the first position to the second positionusing a motor coupled to the heating device. In other examples, themethod comprises providing power to the motor using a DC power sourceelectrically coupled to the motor. In further examples, the methodcomprises providing a non-linear thermal gradient using the heatingdevice. In other examples, the method comprises configuring thechromatography system with a processor. In some instances, the methodcomprises configuring the processor to wireles sly couple to a mobiledevice that receives chromatography information from the system duringthe chromatographic separation.

In certain examples, a method comprising providing a heating deviceconfigured to thermally couple to a chromatography column at an inletsection of the chromatography column in a first position and tothermally couple to the chromatography column at a section downstreamfrom the inlet section in a second position, the heating deviceconfigured to receive the chromatography column during movement of thechromatography column in a longitudinal dimension along the heatingdevice from the first position to the second position during achromatographic separation to provide a thermal gradient to thechromatography column during the chromatographic separation isdescribed. In some examples, the method comprises providing asubstantially linear thermal gradient during the chromatographicseparation by maintaining the heating device at a substantially constanttemperature during the chromatographic separation. In other examples,the method comprises providing a substantially linear thermal gradientduring the chromatographic separation using a cooling device thermallycoupled to the heating device. In further instances, the methodcomprises moving the cooling device during the chromatographicseparation. In additional instances, the method comprises maintainingthe cooling device in a stationary position during the chromatographicseparation. In further instances, the method comprises moving thechromatography column from the first position to the second positionusing a motor coupled to the chromatography column. In some examples,the method comprises providing power to the motor using a DC powersource electrically coupled to the motor. In other examples, the methodcomprises providing a non-linear thermal gradient using the heatingdevice. In certain embodiments, the method comprises configuring thechromatography system with a processor. In further instances, the methodcomprises configuring the processor to wirelessly couple to a mobiledevice that receives chromatography information from the system duringthe chromatographic separation.

In other examples, a method of performing gas chromatography comprisesproviding a heating device configured to thermally couple to innersurfaces of a section of a chromatography column in a first position ofthe heating device and configured to thermally couple to inner surfacesof a different section of the chromatography column in a secondposition, the heating device configured to move from the first positionto the second position to provide a thermal gradient during a gaschromatographic separation. In some instances, the method comprisesproviding a cooling device configured to thermally couple to the heatingdevice, the heating device and cooling device together configured toprovide a substantially linear thermal gradient during the gaschromatographic separation. In further embodiments, the method comprisesproviding the substantially linear thermal gradient during thechromatographic separation by maintaining one end of the heating deviceat a substantially constant temperature during the chromatographicseparation. In some examples, the method comprises moving the coolingdevice during the chromatographic separation. In other instances, themethod comprises maintaining the cooling device in a stationary positionduring the chromatographic separation. In some embodiments, the methodcomprises moving the heating device from the first position to thesecond position using a motor coupled to the heating device. Inadditional configurations, the method comprises providing power to themotor using a DC power source electrically coupled to the motor. In someembodiments, the method comprises providing a non-linear thermalgradient using the heating device. In other examples, the methodcomprises configuring the chromatography system with a processor. Insome examples, the method comprises configuring the processor to wirelessly couple to a mobile device that receives chromatography informationfrom the system during the chromatographic separation.

In certain examples, a method of performing gas chromatography comprisesproviding a heating device configured to thermally couple to innersurfaces of a section of a chromatography column in a first position ofthe heating device and configured to thermally couple to inner surfacesof a different section of the chromatography column in a secondposition, the heating device configured to receive the chromatographycolumn during movement of the chromatography column from the firstposition to the second position to provide a thermal gradient to thechromatography column during a gas chromatographic separation. In someinstances, the method comprises providing a cooling device configured tothermally couple to the heating device, the heating device and coolingdevice together configured to provide a substantially linear thermalgradient during the gas chromatographic separation. In other instances,the method comprises providing the substantially linear thermal gradientduring the chromatographic separation by maintaining one end of theheating device at a substantially constant temperature during thechromatographic separation. In some examples, the method comprisesmoving the cooling device during the chromatographic separation. Inother examples, the method comprises maintaining the cooling device in astationary position during the chromatographic separation. In furtherembodiments, the method comprises moving the chromatography column fromthe first position to the second position using a motor coupled to theheating device. In other examples, the method comprises providing powerto the motor using a DC power source electrically coupled to the motor.In additional examples, the method comprises providing a non-linearthermal gradient using the heating device. In other configurations, themethod comprises configuring the chromatography system with a processor.In further embodiments, the method comprises configuring the processorto wirelessly couple to a mobile device that receives chromatographyinformation from the system during the chromatographic separation.

In certain embodiments, a method of separating two or more analytesusing a thermal gradient comprises providing a heating device configuredto thermally couple to a chromatography column in a column space of achromatography system, in which the heating device is configured tothermally couple to the inlet section of the chromatography column in afirst position and to thermally couple to a section of thechromatography column downstream of the inlet section in a secondposition, and providing instructions for moving the heating device in alongitudinal direction of the chromatography column from the firstposition to the second position to provide a thermal gradient to thechromatography column during a chromatographic separation. In someexamples, the method comprises providing a chromatography columnconfigured to receive the heating device in an inner space formed bycoiling of the chromatography column. In other examples, the methodcomprises providing a motor configured to couple to the heating deviceto control movement of the heating device in the longitudinal direction.In additional examples, the method comprises providing a cooling deviceconfigured to thermally couple to a section of the chromatography columnother than a section where the heating device is thermally coupled, inwhich the heating device and cooling device together are configured toprovide a substantially linear thermal gradient during chromatographicseparation using the chromatography column. In other embodiments, themethod comprises configuring the cooling device as a fan.

In certain embodiments, a method of separating two or more analytesusing a thermal gradient comprises providing a heating device configuredto thermally couple to a chromatography column in a column space of achromatography system, in which the heating device is configured tothermally couple to the inlet section of the chromatography column in afirst position and to thermally couple to a section of thechromatography column downstream of the inlet section in a secondposition, and providing instructions for moving the chromatographycolumn in a longitudinal direction of the chromatography column from thefirst position to the second position to provide a thermal gradient tothe chromatography column during a chromatographic separation. Incertain examples, the method comprises providing a chromatography columnconfigured to receive the heating device in an inner space formed bycoiling of the chromatography column. In some embodiments, the methodcomprises providing a motor configured to couple to the chromatographycolumn to control movement of the chromatography column in thelongitudinal direction. In certain embodiments, the method comprisesproviding a cooling device configured to thermally couple to a sectionof the chromatography column other than a section where the heatingdevice is thermally coupled, in which the heating device and coolingdevice together are configured to provide a substantially linear thermalgradient during chromatographic separation using the chromatographycolumn. In other instances, the method comprises configuring the coolingdevice as a fan.

In certain configurations, a portable chromatography system comprises ahousing sized and arranged to permit carrying of the system by a human,the housing comprising an injector, a column space configured to receivea chromatography column that fluidically couples to the injector toprovide sample injected into the injector to an inlet section of thechromatography column, a heating device configured to thermally coupleto the chromatography column in the column space, in which the heatingdevice is configured to thermally couple to the inlet section of thechromatography column in a first position and to thermally couple to asection of the chromatography column downstream of the inlet section ina second position, in which the heating device is configured to move ina longitudinal direction of the chromatography column from the firstposition to the second position to provide a thermal gradient duringchromatographic separation using the heating device, a detectorconfigured to fluidically couple to the chromatography column at an exitend of the chromatography column to receive analyte from thechromatography column, and a processor electrically coupled to thedetector. The system may also comprise a motor within the housing andcoupled to the heating device and electrically coupled to the processor.The system can also include a DC power source electrically coupled tothe processor and positioned within the housing. In some examples, theDC power source is configured as an electrochemical cell, a fuel cell, asolar cell or a wind turbine configured to provide a direct current. Incertain examples, the heating device is configured to thermally coupleto a column that is one or more of a capillary column, a capillarycolumn bundle and a wafer column.

In certain examples, a portable chromatography system comprises ahousing sized and arranged to permit carrying of the system by a human,the housing comprising an injector, a column space configured to receivea chromatography column that fluidically couples to the injector toprovide sample injected into the injector to an inlet section of thechromatography column, a heating device configured to thermally coupleto the chromatography column in the column space, in which the heatingdevice is configured to thermally couple to the inlet section of thechromatography column in a first position and to thermally couple to asection of the chromatography column downstream of the inlet section ina second position, in which the heating device is configured to receivethe chromatography column during movement of the chromatography columnin a longitudinal direction from the first position to the secondposition to provide a thermal gradient during chromatographic separationusing the heating device, a detector configured to fluidically couple tothe chromatography column at an exit end of the chromatography column toreceive analyte from the chromatography column, and a processorelectrically coupled to the detector. In certain instances, the systemcomprises a motor within the housing and coupled to the chromatographycolumn and electrically coupled to the processor. In other instances,the system comprises a DC power source electrically coupled to theprocessor and positioned within the housing. In some embodiments, the DCpower source is configured as an electrochemical cell, a fuel cell, asolar cell or a wind turbine configured to provide a direct current. Incertain examples, the heating device is configured to thermally coupleto a column that is one or more of a capillary column, a capillarycolumn bundle and a wafer column.

EXAMPLE 1

A system similar to that shown in FIG. 20 (except with only a singleheating device) was used to determine the temperature of a columnwrapped around a glass tube as the heating device was inserted into theglass tube. The heating device (aluminum rod) was held at a temperatureof about 300 deg. C and inserted into the glass tube at a rate of about¼ inch every 10 seconds. The temperature values as a function of thedistance from the end of the glass tube are shown in FIG. 23. Each linerepresents the temperature profile at a selected time. Insertion of theheating device provided a thermal gradient from one end of the tube tothe other.

EXMPLE 2

A system similar to that shown in FIG. 20 was used to determine thetemperature of a column wrapped around a glass tube. Two heating deviceswere used. The cold heating device was held at a temperature of about 50deg. Celsius. The hot heating device was held at a temperature of about300 deg. C. Each heating device was configured as an aluminum rod. Theheating devices were removed/inserted separately at a rate of about ¼inch every 10 seconds. The temperature values as a function of thedistance from the end of the glass tube are shown in FIG. 24. Each linerepresents the temperature profile at a selected time. Use of twoheating devices provided a steeper gradient and permits for moreaccurate control of the cool end of the column.

When introducing elements of the examples disclosed herein, the articles“a,” “an,” “the” and “said” are intended to mean that there are one ormore of the elements. The terms “comprising,” “including” and “having”are intended to be open-ended and mean that there may be additionalelements other than the listed elements. It will be recognized by theperson of ordinary skill in the art, given the benefit of thisdisclosure, that various components of the examples can be interchangedor substituted with various components in other examples.

Although certain aspects, examples and embodiments have been describedabove, it will be recognized by the person of ordinary skill in the art,given the benefit of this disclosure, that additions, substitutions,modifications, and alterations of the disclosed illustrative aspects,examples and embodiments are possible.

The invention claimed is:
 1. A gas chromatography system comprising: afirst heating device configured to thermally couple to a first sectionof a gas chromatography column in a first position of the first heatingdevice and to thermally couple to a second section of the gaschromatography column in a second position of the first heating device,wherein the first heating device is configured to move longitudinallyfrom the first position of the first heating device to the secondposition of the first heating device; and a second heating deviceseparate from the first heating device and configured to moveindependent of the first heating device, wherein the second heatingdevice is configured to thermally couple to a first section of the gaschromatography column in a first position of the second heating deviceand to thermally couple to a second section of the gas chromatographycolumn in a second position of the second heating device, wherein thesecond heating device is configured to move longitudinally from thefirst position of the second heating device to the second position ofthe second heating device.
 2. The gas chromatography system of claim 1,further comprising a respective motor coupled to each of the firstheating device and the second heating device, wherein the motor coupledto the first heating device is configured to move the first heatingdevice from the first position of the first heating device to the secondposition of the first heating device and wherein the motor coupled tothe second heating device is configured to move the second heatingdevice from the first position of the second heating device to thesecond position of the second heating device, wherein the first heatingdevice and the second heating device are configured to provide a thermalgradient to the gas chromatography column.
 3. The gas chromatographysystem of claim 2, wherein the second heating device is configured tocontact the gas chromatography column at the second position of thesecond heating device.
 4. The gas chromatography system of claim 2,further comprising a processor.
 5. The gas chromatography system ofclaim 4, wherein each respective motor is electrically coupled to theprocessor and is configured as a stepper motor.
 6. The gaschromatography system of claim 4, wherein each of the first heatingdevice and the second heating device is independently arranged to beinserted into and out of an interior portion of a coiled chromatographycolumn.
 7. The gas chromatography system of claim 6, wherein each of thefirst heating device and the second heating device is configured as acylinder or rod configured to move into and out of the interior portionof the coiled chromatography column.
 8. The gas chromatography system ofclaim 7, further comprising a DC power source electrically coupled tothe processor and the respective motors.
 9. The gas chromatographysystem of claim 4, wherein a first temperature of the first heatingdevice is selected to permit equilibration of the gas chromatographycolumn at a temperature substantially the same as the first temperaturewhen the first heating device is inserted into an interior portion ofthe gas chromatography column, wherein the processor is configured tomove the inserted, first heating device out of the interior portion ofthe gas chromatography column prior to movement of the second heatingdevice, and wherein after the inserted, first heating device is movedout of the interior portion of the gas chromatography column the secondheating device is inserted longitudinally into the interior portion ofthe gas chromatography column to provide a thermal gradient to the gaschromatography column.
 10. The gas chromatography system of claim 9,wherein the gas chromatography column is wound around a substrate sizedand arranged to receive each of the first heating device and the secondheating device within an interior portion of the substrate.
 11. The gaschromatography system of claim 1, wherein the first heating device isfurther configured to cool the gas chromatography column.
 12. The gaschromatography system of claim 11, further comprising a cooling devicethermally coupled to the first heating device and the gas chromatographycolumn, the cooling device configured to assist in providing a thermalgradient to the gas chromatography column.
 13. The gas chromatographysystem of claim 12, in which the cooling device is configured as one ormore of a fan, a Peltier cooler, and a heatsink.
 14. The gaschromatography system of claim 1, wherein the second heating device isconfigured to move longitudinally into and out of an interior of thefirst heating device.
 15. The gas chromatography system of claim 1,further comprising a detector configured to fluidically couple to thegas chromatography column.
 16. The gas chromatography system of claim 1,wherein the first heating device and the second heating device aretogether configured to provide a non-linear thermal gradient to the gaschromatography column.
 17. A method of providing a thermal gradient to agas chromatography column using the gas chromatography system of claim1, the method comprising: moving the first heating device longitudinallyinto an interior space of a gas chromatography column, wherein the firstheating device is at a first temperature and the first heating deviceremains within the interior space of the gas chromatography column for afirst period to permit equilibration of the gas chromatography column ata temperature substantially the same as the first temperature; removingthe first heating device from the interior space of the gaschromatography column; and moving the second heating devicelongitudinally into the interior space of the gas chromatography column,wherein the second heating device is at a second temperature and ismoved into the interior space of the gas chromatography column toprovide a thermal gradient to the gas chromatography column during a gaschromatographic separation.
 18. The method of claim 17, furthercomprising removing the inserted, second heating device, and moving thefirst heating device back into the interior space of the gaschromatography column to permit equilibration of the gas chromatographycolumn at the temperature substantially the same as the firsttemperature.
 19. The method of claim 17, wherein the second temperatureis 150 degrees Celsius or more higher than the first temperature. 20.The method of claim 17, wherein at least one of the first heating deviceand the second heating device is configured as a hollow cylinder orhollow rod.
 21. The gas chromatography system of claim 1, wherein atleast one of the first heating device and the second heating device isconfigured as a hollow cylinder or hollow rod.
 22. A gas chromatographysystem comprising: a first heating device configured to thermally coupleto a first section of a gas chromatography column in a first position ofthe first heating device and to thermally couple to a second section ofthe gas chromatography column in a second position of the first heatingdevice, wherein the first heating device is configured to movelongitudinally from the first position of the first heating device tothe second position of the first heating device; and a second heatingdevice separate from the first heating device and configured to moveindependent of the first heating device, wherein the second heatingdevice is configured to thermally couple to a first section of the gaschromatography column in a first position of the second heating deviceand to thermally couple to a second section of the gas chromatographycolumn in a second position of the second heating device, wherein thesecond heating device is configured to move longitudinally from thefirst position of the second heating device to the second position ofthe second heating device, wherein each of the first heating device andthe second heating device is configured to thermally couple to innercoil surfaces of a different section of the gas chromatography columnwhen the first heating device is in the second position of the firstheating device and when the second heating device is in the secondposition of the second heating device.
 23. The gas chromatography systemof claim 22, wherein at least one of the first heating device and thesecond heating device is configured as a hollow cylinder or hollow rod.24. A gas chromatography system comprising: a first heating deviceconfigured to thermally couple to a first section of a gaschromatography column in a first position of the first heating deviceand to thermally couple to a second section of the gas chromatographycolumn in a second position of the first heating device, wherein thefirst heating device is configured to move longitudinally from the firstposition of the first heating device to the second position of the firstheating device; and a second heating device separate from the firstheating device, wherein the second heating device is configured tothermally couple to a first section of the gas chromatography column ina first position of the second heating device and to thermally couple toa second section of the gas chromatography column in a second positionof the second heating device, wherein the second heating device isconfigured to move longitudinally from the first position of the secondheating device to the second position of the second heating device, inwhich the first heating device is configured as a cylinder configured tomove through an interior space formed by coiling of the gaschromatography column.
 25. The gas chromatography system of claim 24, inwhich the diameter of the cylinder is sized and arranged to receive andcontact interior surfaces of the gas chromatography column to provide athermal gradient to the gas chromatography column.
 26. The gaschromatography system of claim 24, in which the first heating device isconfigured to thermally couple to two or more gas chromatography columnsto provide a temperature gradient to each of the two or more gaschromatography columns.
 27. The gas chromatography system of claim 24,wherein the second heating device is configured as a hollow cylinder orhollow rod.